강기윤 (인천대학교)

한빛사논문

조회541

인천대학교

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Plant J., Oct 30 2023, | https://doi.org/10.1111/tpj.16517 Rice OsGATA16 is a positive regulator for chlorophyll biosynthesis and chloroplast development

Chaemyeong Lim^1,†, Youngoh Kim^1,†, Yejin Shim¹, Sung-Hwan Cho¹, Tae-Jin Yang¹, Young Hun Song², Kiyoon Kang^3,*and Nam-Chon Paek^1,*

¹Department of Agriculture, Forestry and Bioresources, Plant Genomics and Breeding Institute, Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea,

²Department of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea, and

³Division of Life Sciences, Incheon National University, Incheon, Republic of Korea

^*For correspondence: Kiyoon Kang, Nam-Chon Paek

^†They contributed equally to this work.

Abstract

Chloroplasts are essential organelles in plants that contain chlorophylls and facilitate photosynthesis for growth and development. As photosynthetic efficiency significantly impacts crop productivity, understanding the regulatory mechanisms of chloroplast development has been crucial in increasing grain and biomass production. This study demonstrates the involvement of OsGATA16, an ortholog of Arabidopsis GATA, NITRATE INDUCIBLE, CARBON-METABOLISM INVOLVED (GNC), and GNC-LIKE/CYTOKININ-RESPONSIVE GATA FACTOR 1 (GNL/CGA1), in chlorophyll biosynthesis and chloroplast development in rice (Oryza sativa). The osgata16-1 knockdown mutants produced pale-green leaves, while OsGATA16-overexpressed plants (OsGATA16-OE1) generated dark-green leaves, compared to their parental japonica rice. Reverse transcription and quantitative PCR analysis revealed downregulation of genes related to chloroplast division, chlorophyll biosynthesis, and photosynthesis in the leaves of osgata16-1 and upregulation in those of OsGATA16-OE1. Additionally, in vivo binding assays showed that OsGATA16 directly binds to the promoter regions of OsHEMA, OsCHLH, OsPORA, OsPORB, and OsFtsZ, and upregulates their expression. These findings indicate that OsGATA16 serves as a positive regulator controlling chlorophyll biosynthesis and chloroplast development in rice.

논문정보

형식Research article
게재일2023년 10월 (BRIC 등록일 2023-10-31)
연구진국내 연구진
분야 생명과학 > 식물학

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