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조회863

김민지Min-Ji Kim

경북대학교

Environ. Int., Oct 18 2023, 181 S0160-4120(23)00541-X | https://doi.org/10.1016/j.envint.2023.108268 Interplays between cyanobacterial blooms and antibiotic resistance genes

Min-Ji Kim ^a, Dayun Kang ^b, GyuDae Lee ^a, Kyeongnam Kim ^c, Jinnam Kim ^b, Jae-Ho Shin ^a,d, Seungjun Lee ^b

^aDepartment of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of Korea

^bDepartment of Food Science and Nutrition, Pukyong National University, Busan 48513, Republic of Korea

^cInstitute of Quality and Safety Evaluation of Agricultural Products, Kyungpook National University, Daegu 41566, Republic of Korea

^dNGS Core Facility, Kyungpook National University, Daegu 41566, Republic of Korea

Corresponding authors : Jae-Ho Shin, Seungjun Lee

Abstract

Cyanobacterial harmful algal blooms (cyanoHABs), which are a form of microbial dysbiosis in freshwater environments, are an emerging environmental and public health concern. Additionally, the freshwater environment serves as a reservoir of antibiotic resistance genes (ARGs), which pose a risk of transmission during microbial dysbiosis, such as cyanoHABs. However, the interactions between potential synergistic pollutants, cyanoHABs, and ARGs remain poorly understood. During cyanoHABs, Microcystis and high microcystin levels were dominant in all the nine regions of the river sampled. The resistome, mobilome, and microbiome were interrelated and linked to the physicochemical properties of freshwater. Planktothrix and Pseudanabaena competed with Actinobacteriota and Proteobacteria during cyanoHABs. Forty two ARG carriers were identified, most of which belonged to Actinobacteriota and Proteobacteria. ARG carriers showed a strong correlation with ARGs density, which decreased with the severity of cyanoHAB. Although ARGs decreased due to a reduction of ARG carriers during cyanoHABs, mobile gene elements (MGEs) and virulence factors (VFs) genes increased. We explored the relationship between cyanoHABs and ARGs for potential synergistic interaction. Our findings demonstrated that cyanobacteria compete with freshwater commensal bacteria such as Actinobacteriota and Proteobacteria, which carry ARGs in freshwater, resulting in a reduction of ARGs levels. Moreover, cyanoHABs generate biotic and abiotic stress in the freshwater microbiome, which may lead to an increase in MGEs and VFs. Exploration of the intricate interplays between microbiome, resistome, mobilome, and pathobiome during cyanoHABs not only revealed that the mechanisms underlying the dynamics of microbial dysbiosis but also emphasizes the need to prioritize the prevention of microbial dysbiosis in the risk management of ARGs.

논문정보

형식Research article
게재일2023년 10월 (BRIC 등록일 2023-10-30)
연구진국내 연구진
분야 생명과학 > 응용생물화학

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