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Bioact. Mater., Available online 4 October 2023 | https://doi.org/10.1016/j.bioactmat.2023.07.021 Dragging 3D printing technique controls pore sizes of tissue engineered blood vessels to induce spontaneous cellular assembly

Hun-Jin Jeong ^a,g,1, Hyoryung Nam ^b,1, Jae-Seok Kim ^a, Sungkeon Cho ^d, Hyun-Ha Park ^a, Young-Sam Cho ^c, Hyungkook Jeon ^h, Jinah Jang ^b,d,e,f, Seung-Jae Lee ^c

^aDepartment of Mechanical Engineering, Wonkwang University, 54538, Iksan, Republic of Korea

^bDepartment of Convergence IT Engineering, Pohang University of Science and Technology, 37673, Pohang, Gyeongbuk, Republic of Korea

^cDepartment of Mechanical and Design Engineering, Wonkwang University, 54538, Iksan, Republic of Korea

^dDepartment of Mechanical Engineering, Pohang University of Science and Technology, 37673, Pohang, Gyeongbuk, Republic of Korea

^eSchool of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, 37673, Pohang, Gyeongbuk, Republic of Korea

^fInstitute of Convergence Science, Yonsei University, 03722, Seoul, Republic of Korea

^gRegenerative Engineering Laboratory, Columbia University, 630W 168th ST, New York, 10032, USA

^hDepartment of Manufacturing Systems and Design Engineering, Seoul National University of Science and Technology, 01811, Seoul, Republic of Korea

¹These authors contributed equally to this work.

Corresponding authors: Jinah Jang, Seung-Jae Lee

Abstract

To date, several off-the-shelf products such as artificial blood vessel grafts have been reported and clinically tested for small diameter vessel (SDV) replacement. However, conventional artificial blood vessel grafts lack endothelium and, thus, are not ideal for SDV transplantation as they can cause thrombosis. In addition, a successful artificial blood vessel graft for SDV must have sufficient mechanical properties to withstand various external stresses. Here, we developed a spontaneous cellular assembly SDV (S-SDV) that develops without additional intervention. By improving the dragging 3D printing technique, SDV constructs with free-form, multilayers and controllable pore size can be fabricated at once. Then, The S-SDV filled in the natural polymer bioink containing human umbilical vein endothelial cells (HUVECs) and human aorta smooth muscle cells (HAoSMCs). The endothelium can be induced by migration and self-assembly of endothelial cells through pores of the SDV construct. The antiplatelet adhesion of the formed endothelium on the luminal surface was also confirmed. In addition, this S-SDV had sufficient mechanical properties (burst pressure, suture retention, leakage test) for transplantation. We believe that the S-SDV could address the challenges of conventional SDVs: notably, endothelial formation and mechanical properties. In particular, the S-SDV can be designed simply as a free-form structure with a desired pore size. Since endothelial formation through the pore is easy even in free-form constructs, it is expected to be useful for endothelial formation in vascular structures with branch or curve shapes, and in other tubular tissues such as the esophagus.

논문정보

형식Research article
게재일2023년 10월 (BRIC 등록일 2023-10-31)
연구진국내(교신)+국외 연구진
분야 바이오·의료융합 > 바이오소재

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