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한빛사논문
한빛사논문
- 조회429
Biomaterials, Volume 302, November 2023, 122350 | https://doi.org/10.1016/j.biomaterials.2023.122350
Fusogenic liposomes encapsulating mitochondria as a promising delivery system for osteoarthritis therapy
Hye-Ryoung Kim, Hui Bang Cho, Sujeong Lee, Ji-In Park, Hye Jin Kim, Keun-Hong Park
Laboratory of Nano-regenerative Medicine, Department of Biomedical Science, College of Life Science, CHA University, CHA Biocomplex, 335 Pangyo-ro, Sampyeong-Dong, Bundang-gu, Seongnam-si, 13488, Republic of Korea
Corresponding authors: Hye Jin Kim, Keun-Hong Park
Abstract
Many attempts have been made to use mitochondria (MT) to treat human diseases; however, MT are large, making them difficult to deliver effectively. Therefore, a transfer strategy based on membrane fusion was established. Fusogenic mitochondrial capsules (FMCs) comprising a neutral lipid (PE), a cationic lipid (DOTAP), an aromatic lipid (Liss Rhod PE), and three types of liposome (FMC0, FMC1, and FMC2), were designed and synthesized. The amount of DOTAP, which affects membrane fusion efficiency, differed between FMC preparations. The characteristics of these FMCs were analyzed by DLS, TEM, and AFM, and the encapsulation and fusion efficiency between FMC-MT and FMC-chondrocytes were confirmed by FRET, mtDNA copy number, and CLSM, respectively. Compared with naked MT, delivery of FMCs to chondrocytes was faster and more efficient. Moreover, fusion was a more stable delivery method than endocytosis, as evidenced by reduced induction of mitophagy. In vitro and in vivo experiments revealed that FMCs reduced expression of inflammatory cytokines and MMP13, increased expression of extracellular matrix components, and promoted cartilage regeneration. These findings suggest that FMCs are a highly effective and promising strategy for delivery of MT to promote cartilage regeneration, and highlight their potential as a novel platform for MT transfer therapy.
논문정보
- Research article
- 2023년 11월 (BRIC 등록일 2023-10-23)
- 국내 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
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