한도경 (한국기초과학지원연구원) | 한빛사논문 > 한빛사

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조회419

한도경Do Kyoung Han

한국기초과학지원연구원

Biosens. Bioelectron., Volume 176, 15 March 2021, 112894 | https://doi.org/10.1016/j.bios.2020.112894 Paper-based multiplex analytical device for simultaneous detection of Clostridioides difficile toxins and glutamate dehydrogenase

Do Kyoung Han ^a, Jeonghyun Oh ^a,c, Jaehyeon Lee ^b, Yong Gon Cho ^b, Ju Seong Park ^a,c, Jong Soon Choi ^a,c, Dal Sik Kim ^b, Joseph Kwon ^a

^aResearch Center for Materials Analysis, Korea Basic Science Institute, 169–148, Gwahak-ro, Yuseong-Gu, Daejeon, 34133, Republic of Korea

^bDepartment of Laboratory Medicine, Jeonbuk National University Hospital, Jeonbuk National University Medical School, Jeonju, 54907, Republic of Korea

^cGraduate School of Analytical Science and Technology, Chungnam National University, Daejeon, 34134, Republic of Korea

Corresponding authors: Jong Soon Choi, Dal Sik Kim, Joseph Kwon

Abstract

We report a new paper-based multiplex analytical device (mPAD) for simultaneous screening of three analytes (glutamate dehydrogenase, toxin A, and toxin B) known as biomarkers for Clostridioides difficile infection (CDI). To overcome the limitation of common rapid assays (e.g. lateral flow immunochromatographic and enzyme immunoassays) in terms of multiplexing, sensitivity, simplicity, and ease-of-use, the mPAD is constructed with a three dimensional (3D) configuration of paper components with a multi-channel design. Multiple fluidic paths developed with wax-patterned paper allow the simultaneous detection of glutamate dehydrogenase, toxin A, and toxin B without any cross-reactivity. The 3D fluidic network on the mPAD facilitates a self-operating test procedure for the mixing and addition of amplification reagents with a one-step sliding operation. The results of the multiplex CDI assay can be easily interpreted by the naked eye within 10 min, and are visually intensified over time resulting in up to 3-fold signal amplification. Our device exhibited remarkable analytical performances for the simultaneous detection of three CDI biomarkers, providing a sensitivity of 97%, specificity of 88%, accuracy of 95%, and limits of detection for glutamate dehydrogenase, toxin A, and toxin B of 0.16 ng mL ^-1, 0.09 ng mL ^-1, and 0.03 ng mL ^-1, respectively. These results indicate the high applicability and feasibility of mPAD for multiplex testing for CDI with the advantages of being simple, sensitive, inexpensive, user-friendly, and equipment-free.

논문정보

형식Research article
게재일2021년 03월 (BRIC 등록일 2023-10-20)
연구진국내 연구진
분야 생명과학 > 생화학

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