조장천 (인하대학교)

한빛사논문

조회377

인하대학교

CV File 558 kb

Sci. Data, Oct 17 2023, 10 (1) | https://doi.org/10.1038/s41597-023-02622-0 Metagenome sequencing and recovery of 444 metagenome-assembled genomes from the biofloc aquaculture system

Meora Rajeev^1,2, Ilsuk Jung¹, Yeonjung Lim³, Suhyun Kim³, Ilnam Kang³ & Jang-CheonCho^1,3

¹Department of Biological Sciences and Bioengineering, Inha University, Inharo 100, Incheon 22212, Republic of Korea.

²Institute for Specialized Teaching and Research, Inha University, Inharo 100, Incheon 22212, Republic of Korea.

³Center for Molecular and Cell Biology, Inha University, Inharo 100, Incheon 22212, Republic of Korea.

Corresponding author : Correspondence to Jang-Cheon Cho.

Abstract

Biofloc technology is increasingly recognised as a sustainable aquaculture method. In this technique, bioflocs are generated as microbial aggregates that play pivotal roles in assimilating toxic nitrogenous substances, thereby ensuring high water quality. Despite the crucial roles of the floc-associated bacterial (FAB) community in pathogen control and animal health, earlier microbiota studies have primarily relied on the metataxonomic approaches. Here, we employed shotgun sequencing on eight biofloc metagenomes from a commercial aquaculture system. This resulted in the generation of 106.6 Gbp, and the reconstruction of 444 metagenome-assembled genomes (MAGs). Among the recovered MAGs, 230 were high-quality (≥90% completeness, ≤5% contamination), and 214 were medium-quality (≥50% completeness, ≤10% contamination). Phylogenetic analysis unveiled Rhodobacteraceae as dominant members of the FAB community. The reported metagenomes and MAGs are crucial for elucidating the roles of diverse microorganisms and their functional genes in key processes such as nitrification, denitrification, and remineralization. This study will contribute to scientific understanding of phylogenetic diversity and metabolic capabilities of microbial taxa in aquaculture environments.

논문정보

형식Research article
게재일2023년 10월 (BRIC 등록일 2023-10-19)
연구진국내 연구진
분야 생명과학 > 미생물학

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