상위피인용논문
경희대학교
Ok Kyung Lee a, A Leum Kim a, Dong Ho Seong b, Choul Gyun Lee b, Yeon Tae Jung c, Jin Won Lee c, Eun Yeol Lee a *
aDepartment of Chemical Engineering, Kyung Hee University, Gyeonggi-do 446-701, Republic of Korea
bMarine Bioenergy Research Center, Department of Biological Engineering, Inha University, Incheon 402-751, Republic of Korea
cDepartment of Chemical and Biomolecular Engineering, Sogang University, Seoul 121-742, Republic of Korea
*Corresponding author: correspondence to Eun Yeol Lee
Abstract
Chemo-enzymatic saccharification and bioethanol fermentation of the residual biomass of Dunaliella tertiolecta after lipid extraction for biodiesel production were investigated. HCl-catalyzed saccharification of the residual biomass at 121 °C for 15 min produced reducing sugars with a yield of 29.5% (w/w) based on the residual biomass dry weight. Various enzymes were evaluated for their ability to saccharify the residual biomass. Enzymatic saccharification using AMG 300L produced 21.0 mg/mL of reducing sugar with a yield of 42.0% (w/w) based on the residual biomass at pH 5.5 and 55 °C. Bioethanol was produced from the enzymatic saccharification products without additional pretreatment by Saccharomyces cerevisiae with yields of 0.14 g ethanol/g residual biomass and 0.44 g ethanol/g glucose produced from the residual biomass. The waste residual biomass generated during microalgal biodiesel production could be used for the production of bioethanol to improve the economic feasibility of microalgal biorefinery.
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