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경희대학교

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Bioresour. Technol., Oct 09 2023, 389 S0960-8524(23)01281-6 | https://doi.org/10.1016/j.biortech.2023.129853 Production and characterization of a biodegradable polymer, poly(3-hydroxybutyrate-co-3-hydroxyvalerate), using the type II methanotroph, Methylocystis sp. MJC1.

Ok Kyung Lee ^a,1, Seung Gi Kang ^a,1, Tae-Rim Choi ^b, Yung-Hun Yang ^b, Eun Yeol Lee ^a

^aDepartment of Chemical Engineering (BK21 FOUR Integrated Engineering Program), Kyung Hee University, Yongin-si, Gyeonggi-do 17104, Republic of Korea

^bDepartment of Biological Engineering, College of Engineering, Konkuk University, Seoul 05029, Republic of Korea

¹These authors contributed equally to this work.

Corresponding author : Eun Yeol Lee

Abstract

The production of polyhydroxyalkanoates (PHAs) through the biological conversion of methane is a promising solution to address both methane emissions and plastic waste. Type II methanotrophs naturally accumulate a representative PHA, poly(3-hydroxybutyrate) (PHB), using methane as the sole carbon source. In this study, we aimed to produce poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV copolymer) with improved properties compared with PHB, using the type II methanotroph, Methylocystis sp. MJC1. We optimized the pH, valerate concentration, and valerate supply time in a one-step cultivation process using a gas bioreactor to enhance PHBV copolymer production yield and the 3-hydroxyvalerate (3HV) molar fraction. Under the optimal conditions, the biomass reached 21.3 g DCW/L, and PHBV copolymer accumulation accounted for 41.9 % of the dried cell weight, with a 3HV molar fraction of 28.4 %. The physicochemical properties of the purified PHBV copolymer were characterized using NMR, FTIR, TGA, DSC, and GPC.

논문정보

형식Research article
게재일2023년 10월 (BRIC 등록일 2023-10-13)
연구진국내 연구진
분야 생명과학 > 생물공학

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