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Johns Hopkins University

Adv. Healthc. Mater., Oct 11 2023 | https://doi.org/10.1002/adhm.202301124 NanoIEA: A Nanopatterned Interdigitated Electrode Array-based Impedance Assay for Real-time Measurement of Aligned Endothelial Cell Barrier Functions.

Jong Seob Choi ^1,2, Hyun Myung Doo ³, Byunggik Kim ⁴, Su Han Lee ⁵, Sang-Keun Sung ⁵, Gwangjun Go ¹, Allister Suarez ¹, Yeseul Kim ⁶, Byung Mook Weon ⁶, Byung-Ok Choi ^3,7, Hyung Jin Kim ⁸, Deok-Ho Kim ¹

¹Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, 21205, United States.

²Division of Advanced Materials Engineering, Kongju National University, Cheonan, Chungnam, 31080, South Korea.

³Department of Health Sciences and Technology, SAIHST, Sungkyunkwan University, Seoul, 06351, South Korea.

⁴Department of Mechanical Engineering, Johns Hopkins University, Baltimore, MD, 21218, United States.

⁵Digital Health Care Research Center, Gumi Electronics, and Information Technology Research Institute (GERI), Gumi, Gyeongbuk, 39253, South Korea.

⁶Soft Matter Physics Laboratory, School of Advanced Materials Science and Engineering, SKKU Advanced Institute of Nanotechnology (SAINT), Sungkyunkwan University, Suwon, 16419, South Korea.

⁷Department of Neurology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, 06351, South Korea.

⁸School of Electrical & Electronic Engineering, Ulsan College, Ulsan, 44610, South Korea.

^†J.S. Choi and H.M. Doo contributed equally to this work.

CORRESPONDING AUTHOR : Deok-Ho Kim

Abstract

A nanopatterned interdigitated electrode array (nanoIEA)-based impedance assay is developed for quantitative real-time measurement of aligned endothelial cell (EC) barrier functions in vitro. A bioinspired poly (L-DOPA) coating is applied to improve the human brain EC adhesion onto the Nafion nanopatterned surfaces. We found that a poly (L-DOPA)-coated Nafion grooved nanopattern makes the human brain ECs orient along the nanopattern direction. Aligned human brain ECs on Nafion nanopatterns exhibit increased expression of genes encoding tight and adherens junction proteins. Aligned human brain ECs also have enhanced impedance and resistance versus unaligned ones. Treatment with a GSK3 inhibitor (GSK3i) further increases impedance and resistance, suggesting synergistic effects occur on the cell-cell tightness of in vitro human brain ECs via a combination of anisotropic matrix nanotopography and GSK3i treatment. We found that this enhanced cell-cell tightness of the combined approach is accompanied by increased expression of claudin protein. Our data demonstrate that the proposed nanoIEA assay integrated with poly (L-DOPA)-coated Nafion nanopatterns and interdigitated electrode arrays can make not only biomimetic aligned ECs, but also enable real-time measurement of the enhanced barrier functions of aligned ECs via tighter cell-cell junctions. This article is protected by copyright. All rights reserved.

논문정보

형식Research article
게재일2023년 10월 (BRIC 등록일 2023-10-18)
연구진국내+국외 연구진
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