황석연 (서울대학교)

한빛사논문

조회449

서울대학교

CV File 301 kb

ACS Nano, Oct 04 2023, | https://doi.org/10.1021/acsnano.3c06846 Single-Walled Carbon Nanotube-Guided Topical Skin Delivery of Tyrosinase to Prevent Photoinduced Damage.

Junghyeon Ko 1¶, Min Jeong Lee 2¶, Woojin Jeong 2, Subin Choi 3, Eunhye Shin 1, Young-Hyeon An 1,3,4, Hyeon-Jin Kim 1, Uk-Jae Lee 1,4, Byung-Gee Kim 1,4, Seon-Yeong Kwak 2,4, Nathaniel S Hwang 1,3,4,5

1School of Chemical and Biological Engineering, Institute of Chemical Processes, Seoul National University, Seoul 08826, Republic of Korea.

2Department of Agriculture, Forestry and Bioresources, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea.

3Interdisciplinary Program in Bioengineering, Seoul National University, Seoul 08826, Republic of Korea.

4BioMax/N-Bio Institute, Seoul National University, Seoul 08826, Republic of Korea.

5Institute of Engineering Research, Seoul National University, Seoul 08826, Republic of Korea.

¶(J.K., M.J.L.)These authors contributed equally.

Corresponding Authors : Seon-Yeong Kwak, Nathaniel S. Hwang

Abstract

When the skin is exposed to ultraviolet radiation (UV), it leads to the degradation of the extracellular matrix (ECM) and results in inflammation. Subsequently, melanocytes are triggered to induce tyrosinase-mediated melanin synthesis, protecting the skin. Here, we introduce a proactive approach to protect the skin from photodamage via the topical delivery of Streptomyces avermitilis-derived tyrosinase (SaTy) using single-walled carbon nanotube (SWNT). Utilizing a reverse electrodialysis (RED) battery, we facilitated the delivery of SaTy-SWNT complexes up to depths of approximately 300 μm, as analyzed by using confocal Raman microscopy. When applied to ex vivo porcine skin and in vivo albino mouse skin, SaTy-SWNT synthesized melanin, resulting in 4-fold greater UV/vis absorption at 475 nm than in mice without SaTy-SWNT. The synthesized melanin efficiently absorbed UV light and alleviated skin inflammation. In addition, the densification of dermal collagen, achieved through SaTy-mediated cross-linking, reduced photoinduced wrinkles by 66.3% in the affected area. Our findings suggest that SWNT-mediated topical protein delivery holds promise in tissue engineering applications.

논문정보

형식Research article
게재일2023년 10월 (BRIC 등록일 2023-10-11)
연구진국내 연구진
분야 바이오·의료융합 > 바이오소재

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