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Plant Biotechnol. J., Oct 04 2023 | https://doi.org/10.1111/pbi.14188
Prime editing: Mechanism insight and recent applications in plants.
Tien V. Vu1,*,†, Ngan Thi Nguyen1,†, Jihae Kim1, Jong Chan Hong1 and Jae-Yean Kim1,2,3,*
1Division of Applied Life Science (BK21 Four Program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University,Jinju, Korea
2Division of Life Science, Gyeongsang National University, Jinju, Korea
3Nulla Bio Inc., Jinju, Korea
†These authors contributed equally to this article.
*CORRESPONDING AUTHORS: Tien V. Vu, Jae-Yean Kim
Abstract
Prime editing (PE) technology utilizes an extended prime editing guide RNA (pegRNA) to direct a fusion peptide consisting of nCas9 (H840) and reverse transcriptase (RT) to a specific location in the genome. This enables the installation of base changes at the targeted site using the extended portion of the pegRNA through RT activity. The resulting product of the RT reaction forms a 3' flap, which can be incorporated into the genomic site through a series of biochemical steps involving DNA repair and synthesis pathways. PE has demonstrated its effectiveness in achieving almost all forms of precise gene editing, such as base conversions (all types), DNA sequence insertions and deletions, chromosomal translocation and inversion and long DNA sequence insertion at safe harbour sites within the genome. In plant science, PE could serve as a groundbreaking tool for precise gene editing, allowing the creation of desired alleles to improve crop varieties. Nevertheless, its application has encountered limitations due to efficiency constraints, particularly in dicotyledonous plants. In this review, we discuss the step-by-step mechanism of PE, shedding light on the critical aspects of each step while suggesting possible solutions to enhance its efficiency. Additionally, we present an overview of recent advancements and future perspectives in PE research specifically focused on plants, examining the key technical considerations of its applications.
논문정보
- Review Paper
- 2023년 10월 (BRIC 등록일 2023-10-10)
- 국내 연구진
- 생명과학 > 생물공학
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