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Tayyab IslamTayyab Islam

UNIST

Bioresour. Technol., Available online 1 October 2023 | https://doi.org/10.1016/j.biortech.2023.129814 Metabolic engineering of Escherichia coli for enhanced production of 1,3-butanediol from glucose.

Tayyab Islam ^a, Thuan Phu Nguyen-Vo ^a,1, Seunghyun Cho ^a, Junhak Lee^a,b, Vivek Kumar Gaur ^a, Sunghoon Park ^a

^aSchool of Energy and Chemical Engineering, Ulsan National Institute of Science and Technology, Ulsan 44919, Republic of Korea

^bR&D Center, ACTIVON Co., Ltd., Cheongju 28104, Republic of Korea

¹Presently: Department of Chemical and Biochemical Engineering, North Carolina State University, Raleigh, NC, 27606, USA.

Corresponding author : Sunghoon Park

Abstract

1,3-Butanediol (1,3-BDO) finds versatile applications in the cosmetic, chemical, and food industries. This study focuses on the metabolic engineering of Escherichia coli K12 to achieve efficient production of 1,3-BDO from glucose via acetoacetyl-CoA, 3-hydroxybutyryl-CoA, and 3-hydroxybutyraldehyde. The accumulation of an intermediary metabolite (pyruvate) and a byproduct (3-hydroxybutyric acid) was reduced by disruption of the negative transcription factor (PdhR) for pyruvate dehydrogenase complex (PDHc) and employing an efficient alcohol dehydrogenase (YjgB), respectively. Additionally, to improve NADPH availability, carbon flux was redirected from the Embden-Meyerhof-Parnas (EMP) pathway to the Entner-Doudoroff (ED) pathway. One resulting strain achieved a record-high titer of 790 mM (∼71.1 g/L) with a yield of 0.65 mol/mol for optically pure (R)-1,3-BDO, with an enantiomeric excess (e.e.) value of 98.5 %. These findings are useful in the commercial production of 1,3-BDO and provide valuable insights into the development of an efficient cell factory for other acetyl-CoA derivatives.

논문정보

형식Research article
게재일2023년 10월 (BRIC 등록일 2023-10-17)
연구진국내 연구진
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