안주성 (고려대학교) | 한빛사논문 > 한빛사

한빛사논문

조회644

고려대학교

CV File 458 kb

Angew. Chem.-Int. Edit., Oct 02 2023, 62 (40) | https://doi.org/10.1002/anie.202310408 A Fluorescent Probe for Investigating the Role of Biothiols in Signaling Pathways Associated with Cerebral Ischemia-Reperfusion Injury.

Yutao Yang ^1,2+, Ming Ma ¹, Lei Shen 1^,Jusung An³⁺, Eunji Kim ³, Hongmei Liu^1*, Ming Jin ¹, Shuxiang Wang ¹, Jinchao Zhang¹, Jong Seung Kim ^3*, Caixia Yin ²^*

¹Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of the Ministry of Education, Chemical Biology Key Laboratory of Hebei Province, College of Chemistry and Materials Science, Hebei University, Baoding, 071002, P. R. China.

²Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Molecular Science, Shanxi University, Taiyuan, 030006, China.

³Department of Chemistry, Korea University, Seoul, 02841, Korea.

⁺These authors contributed equally to this work.

^*CORRESPONDING AUTHOR : Hongmei Liu, Prof. Jong Seung Kim, Prof. Caixia Yin

Abstract

Cerebral ischemia-reperfusion injury (CIRI) is intimately associated with the redox regulation of biothiol, a crucial antioxidant marker that precludes the onset of ROS. We designed a novel fluorescent probe, DCI-Ac-Py, showing various physicochemical properties, such as high selectivity, exceptional signal-to-noise ratio, near-infrared (NIR) optical window, and blood-brain barrier (BBB) penetrability, for detecting biothiols in the brain. The picolinate serves as a specific recognition group that is rapidly activated by biothiol and undergoes nucleophilic substitution with the adjacent acrylic ester to yield the desired NIR probe. Additionally, the probe's lipid solubility is improved through the inclusion of halogen atoms, which aids in penetrating the BBB. Using DCI-Ac-Py, we investigated changes of biothiols in vivo in the brains of mice during CIRI. We found that biothiol-mediated NF-kB classical (P65-related) and nonclassical (RelB-related) pathways contribute to abundant ROS production induced by CIRI and that biothiols are involved in redox regulation. These findings provide new insights into the study of CIRI and shed light on the physiological and pathological mechanisms of biothiols in the brain.

논문정보

형식Research article
게재일2023년 10월 (BRIC 등록일 2023-10-04)
연구진국내(교신)+국외 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

댓글 작성 로그인

CV 열람하기

연락처 보기