강지희 (가천대학교 약학대학)

한빛사논문

조회465

가천대학교 약학대학

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Acta Pharm. Sin. B, Sep 2023, 13 (9) | https://doi.org/10.1016/j.apsb.2023.03.023 Brain endothelial cell-derived extracellular vesicles with a mitochondria-targeting photosensitizer effectively treat glioblastoma by hijacking the blood‒brain barrier.

Thuy Giang Nguyen Cao^{a †}, Ji Hee Kang ^{b †}, Su Jin Kang ^{a †}, Quan Truong Hoang^a, Han Chang Kang ^c, Won Jong Rhee ^a,d, Yu Shrike Zhang ^e, Young Tag Ko ^b, Min Suk Shim ^a

^aDivision of Bioengineering, Incheon National University, Incheon 22012, Republic of Korea

^bCollege of Pharmacy, Gachon University, Incheon 21936, Republic of Korea

^cDepartment of Pharmacy, Integrated Research Institute of Pharmaceutical Sciences, and BK21 PLUS Team for Creative Leader Program for Pharmacomics-based Future Pharmacy, College of Pharmacy, the Catholic University of Korea, Gyeonggi-do 14662, Republic of Korea

^dResearch Center for Bio Materials & Process Development, Incheon National University, Incheon 22012, Republic of Korea

^eDivision of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139, USA

^†These authors made equal contributions to this work.

Corresponding authors : Won Jong Rhee, Yu Shrike Zhang, Young Tag Ko, Min Suk Shim

Abstract

Glioblastoma (GBM) is the most aggressive malignant brain tumor and has a high mortality rate. Photodynamic therapy (PDT) has emerged as a promising approach for the treatment of malignant brain tumors. However, the use of PDT for the treatment of GBM has been limited by its low blood‒brain barrier (BBB) permeability and lack of cancer-targeting ability. Herein, brain endothelial cell-derived extracellular vesicles (bEVs) were used as a biocompatible nanoplatform to transport photosensitizers into brain tumors across the BBB. To enhance PDT efficacy, the photosensitizer chlorin e6 (Ce6) was linked to mitochondria-targeting triphenylphosphonium (TPP) and entrapped into bEVs. TPP-conjugated Ce6 (TPP-Ce6) selectively accumulated in the mitochondria, which rendered brain tumor cells more susceptible to reactive oxygen species-induced apoptosis under light irradiation. Moreover, the encapsulation of TPP-Ce6 into bEVs markedly improved the aqueous stability and cellular internalization of TPP-Ce6, leading to significantly enhanced PDT efficacy in U87MG GBM cells. An in vivo biodistribution study using orthotopic GBM-xenografted mice showed that bEVs containing TPP-Ce6 [bEV(TPP-Ce6)] substantially accumulated in brain tumors after BBB penetration via transferrin receptor-mediated transcytosis. As such, bEV(TPP-Ce6)-mediated PDT considerably inhibited the growth of GBM without causing adverse systemic toxicity, suggesting that mitochondria are an effective target for photodynamic GBM therapy.

논문정보

형식Research article
게재일2023년 09월 (BRIC 등록일 2023-10-04)
연구진국내(교신)+국외 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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