김나현 (울산대학교 의과대학, 서울아산병원) | 한빛사논문 > 한빛사

한빛사논문

조회509

김나현Na Hyun Kim

울산대학교 의과대학, 서울아산병원

Exp. Mol. Med., Oct 02 2023 | https://doi.org/10.1038/s12276-023-01099-6 Systemic antibiotics cause deterioration of emphysema associated with exaggerated inflammation and autophagy.

Na Hyun Kim¹, Bo-Yun Choi², Eun Sil Kim³, Su Jung Kim⁴, Jeong Yeon Hong⁴, Sun-Hee Heo¹, Jin-Yong Jeong³, Kyunggon Kim⁴, Hyun Ju Yoo³, Woo Jun Sul² and Sei Won Lee¹

¹Department of Pulmonary and Critical Care Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea.

²Department of Systems Biotechnology, Chung-Ang University, Anseong, Gyeonggi-do, Republic of Korea.

³Department of Convergence Medicine, Asan Institute for Life Sciences, Asan Medical Center and Department of Microbiology, University of Ulsan College of Medicine, Seoul, Republic of Korea.

⁴Department of Convergence Medicine, Asan Medical Center, Department of Digital Medicine, University of Ulsan, College of Medicine, Seoul, Republic of Korea.

Corresponding author : Correspondence to Sei Won Lee.

Abstract

The interaction between the microbial environment and the host is important for immune homeostasis. Recent research suggests that microbiota dysbiosis can be involved in respiratory diseases. Emphysema is a chronic inflammatory disease, but it is unclear whether dysbiosis caused by antibiotics can affect disease progression. Here, we tried to elucidate the effect of systemic antibiotics on smoking-exposed emphysema models. In this study, the antibiotic mixture caused more alveolar destruction and airspace expansion in the smoking group than in the smoking only or control groups. This emphysema aggravation as a result of antibiotic exposure was associated with increased levels of inflammatory cells, IL-6, IFNγ and protein concentrations in bronchoalveolar lavage fluid. Proteomics analysis indicated that autophagy could be involved in antibiotic-associated emphysema aggravation, and increased protein levels of LC3B, atg3, and atg7 were identified by Western blotting. In microbiome and metabolome analyses, the composition of the gut microbiota was different with smoking and antibiotic exposure, and the levels of short-chain fatty acids (SCFAs), including acetate and propionate, were reduced by antibiotic exposure. SCFA administration restored emphysema development with reduced inflammatory cells, IL-6, and IFNγ and decreased LC3B, atg3, and atg7 levels. In conclusion, antibiotics can aggravate emphysema, and inflammation and autophagy may be associated with this aggravation. This study provides important insight into the systemic impact of microbial dysbiosis and the therapeutic potential of utilizing the gut microbiota in emphysema.

논문정보

형식Research article
게재일2023년 10월 (BRIC 등록일 2023-10-04)
연구진국내 연구진
분야 의약학 > 면역의학

댓글 작성 로그인

CV 열람하기

연락처 보기