오석배 (서울대학교)

한빛사 논문

조회174

서울대학교

CV File 242 kb

Int. J. Oral Sci., Sep 25 2023, 15 (1) | https://doi.org/10.1038/s41368-023-00246-z Transcriptional profiling of dental sensory and proprioceptive trigeminal neurons using single-cell RNA sequencing.

Pa Reum Lee^1,5, Jihoon Kim², Heather Lynn Rossi³, Sena Chung¹, Seung Yub Han⁴, Junhyong Kim^2,4and Seog Bae Oh¹

¹Department of Neurobiology and Physiology, School of Dentistry and Dental Research Institute, Seoul National University, Seoul, Republic of Korea

²Genomics and Computational Biology Graduate Group, University of Pennsylvania, Philadelphia, PA, USA

³Department of Pathobiology, University of Pennsylvania, Philadelphia, PA, USA

⁴Department of Biology, University of Pennsylvania, Philadelphia, PA, USA

⁵Present address: Brain Science Institute, Korea Institute of Science and Technology (KIST), Seoul, Republic of Korea

These authors contributed equally: Pa Reum Lee, Jihoon Kim

Correspondence: Junhyong Kim or Seog Bae Oh

Abstract

Dental primary afferent (DPA) neurons and proprioceptive mesencephalic trigeminal nucleus (MTN) neurons, located in the trigeminal ganglion and the brainstem, respectively, are essential for controlling masticatory functions. Despite extensive transcriptomic studies on various somatosensory neurons, there is still a lack of knowledge about the molecular identities of these populations due to technical challenges in their circuit-validated isolation. Here, we employed high-depth single-cell RNA sequencing (scRNA-seq) in combination with retrograde tracing in mice to identify intrinsic transcriptional features of DPA and MTN neurons. Our transcriptome analysis revealed five major types of DPA neurons with cell type-specific gene enrichment, some of which exhibit unique mechano-nociceptive properties capable of transmitting nociception in response to innocuous mechanical stimuli in the teeth. Furthermore, we discovered cellular heterogeneity within MTN neurons that potentially contribute to their responsiveness to mechanical stretch in the masseter muscle spindles. Additionally, DPA and MTN neurons represented sensory compartments with distinct molecular profiles characterized by various ion channels, receptors, neuropeptides, and mechanoreceptors. Together, our study provides new biological insights regarding the highly specialized mechanosensory functions of DPA and MTN neurons in pain and proprioception.

논문정보

형식Research article
게재일2023년 09월 (BRIC 등록일 2023-09-27)
연구진국내(교신)+국외 연구진
분야 의약학 > 신경의학

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