이민 (University of California, Los Angeles)

한빛사 논문

조회132

University of California, Los Angeles

CV File 216 kb

Biomaterials, Sep 20 2023, 302 S0142-9612(23)00343-5 | https://doi.org/10.1016/j.biomaterials.2023.122335 Complementary modulation of BMP signaling improves bone healing efficiency.

Jiabing Fan ^a,b, Xiao Zhang ^a, Minjee Kang ^a, Chung-Sung Lee ^a,c, Lauren Kim ^a, Danny Hadaya ^d, Tara L. Aghaloo ^d, Min Lee ^a,e

^aDivision of Advanced Prosthodontics, School of Dentistry, University of California, Los Angeles, CA, 90095, USA

^bDepartment of Pharmaceutical Sciences, School of Pharmacy and Health Professions, University of Maryland Eastern Shore, Princess Anne, MD, 21853, USA

^cDepartment of Pharmaceutical Engineering, Soonchunhyang University, Asan, Chungcheongnam-do, 31538, Republic of Korea

^dDivision of Diagnostic and Surgical Sciences, School of Dentistry, University of California, Los Angeles, CA, 90095, USA

^eDepartment of Bioengineering, University of California, Los Angeles, CA, 90095, USA

Corresponding authors: Tara L. Aghaloo, Min Lee

Abstract

The bone morphogenetic protein (BMP) signaling pathway plays a crucial role in bone development and regeneration. While BMP-2 is widely used as an alternative to autograft, its clinical application has raised concerns about adverse side effects and deteriorated bone quality. Therefore, there is a need to develop more sophisticated approaches to regulate BMP signaling and promote bone regeneration. Here, we present a novel complementary strategy that targets both BMP antagonist noggin and agonist Trb3 to enhance bone defect repair without the application of exogenous BMP-2. In vitro studies showed that overexpression of Trb3 with simultaneous noggin suppression significantly promotes osteogenic differentiation of mesenchymal stem cells. This was accompanied by increased BMP/Smad signaling. We also developed sterosome nanocarriers, a non-phospholipid liposomal system, to achieve non-viral mediated noggin suppression and Trb3 overexpression. The gene-loaded sterosomes were integrated onto an apatite-coated polymer scaffold for in vivo calvarial defect implantation, resulting in robust bone healing compared to BMP-2 treatments. Our work provides a promising alternative for high-quality bone formation by regulating expression of BMP agonists and antagonists.

논문정보

형식Research article
게재일2023년 09월 (BRIC 등록일 2023-09-27)
연구진국내+국외 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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