한빛사 논문
- 조회148
Mater. Today Adv., Aug 2023, 19 100408 | https://doi.org/10.1016/j.mtadv.2023.100408
Inkjet-printed morphogenesis of tumor-stroma interface using bi-cellular bioinks of collagen-poly(N-isopropyl acrylamide-co-methyl methacrylate) mixture.
Cih Cheng 1, Naomi Deneke 2, Hye-Ran Moon 1, Sae Rome Choi 1, Natalia Ospina-Muñoz 1, Bennett D Elzey 3,4, Chelsea S Davis 2, George T-C Chiu 1, Bumsoo Han 1,3,5
1School of Mechanical Engineering, Purdue University, West Lafayette, IN, USA.
2School of Materials Engineering, Purdue University, West Lafayette, IN, USA.
3Purdue Institute for Cancer Research, Purdue University, West Lafayette, IN, USA.
4Department of Comparative Pathobiology, Purdue University, West Lafayette, IN, USA.
5Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN, USA.
Corresponding author : Bumsoo Han
Abstract
Recent advances in biomaterials and 3D printing/culture methods enable various tissue-engineered tumor models. However, it is still challenging to achieve native tumor-like characteristics due to lower cell density than native tissues and prolonged culture duration for maturation. Here, we report a new method to create tumoroids with a mechanically active tumor-stroma interface at extremely high cell density. This method, named "inkjet-printed morphogenesis" (iPM) of the tumor-stroma interface, is based on a hypothesis that cellular contractile force can significantly remodel the cell-laden polymer matrix to form densely-packed tissue-like constructs. Thus, differential cell-derived compaction of tumor cells and cancer-associated fibroblasts (CAFs) can be used to build a mechanically active tumor-stroma interface. In this methods, two kinds of bioinks are prepared, in which tumor cells and CAFs are suspended respectively in the mixture of collagen and poly (N-isopropyl acrylamide-co-methyl methacrylate) solution. These two cellular inks are inkjet-printed in multi-line or multi-layer patterns. As a result of cell-derived compaction, the resulting structure forms tumoroids with mechanically active tumor-stroma interface at extremely high cell density. We further test our working hypothesis that the morphogenesis can be controlled by manipulating the force balance between cellular contractile force and matrix stiffness. Furthermore, this new concept of "morphogenetic printing" is demonstrated to create more complex structures beyond current 3D bioprinting techniques.
논문정보
- Research article
- 2023년 08월 (BRIC 등록일 2023-09-27)
- 국외 연구진
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