김주곤 (서울대학교) | 한빛사논문 > 한빛사

한빛사논문

조회481

서울대학교

CV File 537 kb

Plant Physiol., Sep 19 2023, kiad504 | https://doi.org/10.1093/plphys/kiad504 The rice NUCLEAR FACTOR-YA5 and MICRORNA169a module promotes nitrogen utilization during nitrogen deficiency.

Jun Sung Seo ¹, Sung Hwan Kim ², Jae Sung Shim ^1,3, Taeyoung Um ¹, Nuri Oh ², Taehyeon Park ³, Youn Shic Kim ¹, Se-Jun Oh ⁴, Ju-Kon Kim ^1,3,4

¹GreenBio Science & Technology, Seoul National University, Pyeongchang 25354, Korea.

²Crop Biotechnology Institute, Graduate School of International Agricultural Technology, Seoul National University, Pyeongchang 25354, Korea.

³School of Biological Sciences and Technology, Chonnam National University, Gwangju 61186, Korea.

⁴LaSemilla Co. Ltd, Pyeongchang 25354, Korea.

Corresponding author: Ju-Kon Kim

Abstract

Nitrogen (N) is essential for plant growth and development. Therefore, understanding its utilization is essential for improving crop productivity. However, much remains to be learned about plant N sensing and signaling. Here, rice (Oryza sativa) NUCLEAR FACTOR-YA5 (OsNF-YA5) expression was tightly regulated by N status and induced under N-deficient conditions. Over-expression (OE) of OsNF-YA5 in rice resulted in increased chlorophyll levels and delayed senescence compared to control plants under normal N conditions. Agronomic traits were significantly improved in OE plants and impaired in knockout mutants under N-deficient conditions. Using a dexamethasone-inducible system, we identified the putative targets of OsNF-YA5 that include amino acid, nitrate/peptide transporters, and NITRATE TRANSPORTER 1.1A (OsNRT1.1A), which functions as a key transporter in rice. OsNF-YA5 directly enhanced OsNRT1.1A expression and N uptake rate under N-deficient conditions. Besides, overexpression of OsNF-YA5 also enhanced the expression of GLUTAMINE SYNTHETASE 1/2 (GS1/2) and GLUTAMINE OXOGLUTARATE AMINOTRANSFERASE 1/2 (GOGAT1/2), increasing free amino acid contents under N-deficient conditions. Osa-miR169a expression showed an opposite pattern with OsNF-YA5 depending on N status. Further analysis revealed that osa-miR169a negatively regulates OsNF-YA5 expression and N utilization, demonstrating that an OsNF-YA5/osa-miR169a module tightly regulates rice N utilization for adaptation to N status.

논문정보

형식Research article
게재일2023년 09월 (BRIC 등록일 2023-09-21)
연구진국내 연구진
분야 생명과학 > 식량작물 및 원예작물

댓글 작성 로그인

CV 열람하기

연락처 보기