선경호 (KAIST)

한빛사논문

조회255

선경호Gyeongho Seon

KAIST

Chem. Eng. J., Available online 8 August 2022 | https://doi.org/10.1016/j.cej.2022.138543 Development of an integrated biomass refinery process for whole cell biomass utilization of Chlorella sp. ABC-001

Gyeongho Seon ^a,1, Minsik Kim^b,1, Yong Wook Lee ^a, Jun Muk Cho^c, Hogi Kim ^a, Won-Kun Park ^d, Yong Keun Chang^a,e

^aDepartment of Chemical & Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea

^bCell Factory Research Center, Korea Research Institute of Biosciences and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea

^cClean Air Center, Korea Institute of Science and Technology, Seoul 02792, Republic of Korea

^dDepartment of Chemistry and Energy Engineering, Sangmyung University, Seoul 03016, Republic of Korea

^eAdvanced Biomass R&D Center, Daejeon 34141, Republic of Korea

¹These authors contributed equally to this work.

Corresponding authors : Won-Kun Park, Yong Keun Chang

Abstract

Microalgae are a promising source of biomass for the production of biofuels, but their use is currently infeasible because of changes in the biofuel market and the lack of an optimized refinery process. The biomass of oleaginous microalgae consists of up to 50% lipids and 50% carbohydrates and proteins, so the biorefinery process must be able to use whole cell biomass. In this work, an alternative process to the standard biorefinery approach of lipid extraction and treatment of lipid-extracted residue was developed and tested using oleaginous Chlorella sp. ABC-001. First, a hydrolysis process was optimized to achieve cell lysis. At 170 °C and 0.1 N H2SO4, 89.0% of total carbohydrates were hydrolyzed to monosaccharides within 4 min. Then, a volume reduction process (brief and mild centrifugation) was applied to reduce the extraction time and the use of organic solvent. By reducing the aqueous phase from the hydrolysate, all lipids were extracted by vortexing with just a quarter of the original volume (10 mL) within 20 min. Finally, after extraction the aqueous phase was utilized as the sole growth medium for ethanol production by Saccharomyces cerevisiae KL17. The resulting ethanol yield was 0.39 g ethanol/g sugar, comparable to that achieved using conventional yeast-peptone-dextrose (YPD) medium (0.41 g ethanol/g sugar). Furthermore, relative to the conventional process, techno-economic analysis confirmed that the newly developed process decreased operational expenditures by 82.7% and decreased capital expenditures by 70.1%.

논문정보

형식Research article
게재일2023년 08월 (BRIC 등록일 2023-09-19)
연구진국내 연구진
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