김정희 (Johns Hopkins University)

한빛사 논문

조회156

김정희Jeong Hee Kim

Johns Hopkins University

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Sci. Adv., Sep 15 2023, 9 (37) | https://doi.org/10.1126/sciadv.adg6231 Noninvasive morpho-molecular imaging reveals early therapy-induced senescence in human cancer cells.

Arianna Bresci ^{1 †}, Jeong Hee Kim ^{2 †}, Silvia Ghislanzoni ³, Francesco Manetti ¹, Lintong Wu ², Federico Vernuccio ¹, Chiara Ceconello ¹, Salvatore Sorrentino ¹, Ishan Barman ^{2 4 5 *}, Italia Bongarzone ^{3 *}, Giulio Cerullo ^{1 6}, Renzo Vanna ⁶, Dario Polli ^{1 6 *}

¹Department of Physics, Politecnico di Milano, Milan, Italy.
²Department of Mechanical Engineering, Johns Hopkins University, Baltimore, MD, USA.
³Department of Advanced Diagnostics, Fondazione IRCCS Istituto Nazionale Tumori, Milan, Italy.
⁴Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
⁵The Russell H. Morgan Department of Radiology and Radiological Science, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
⁶CNR-Institute for Photonics and Nanotechnologies (CNR-IFN), Milan, Italy.

^†These authors contributed equally to this work.
^*Corresponding author: correspondence to Ishan Barman, Italia Bongarzone or Dario Polli

Abstract

Anticancer therapy screening in vitro identifies additional treatments and improves clinical outcomes. Systematically, although most tested cells respond to cues with apoptosis, an appreciable portion enters a senescent state, a critical condition potentially driving tumor resistance and relapse. Conventional screening protocols would strongly benefit from prompt identification and monitoring of therapy-induced senescent (TIS) cells in their native form. We combined complementary all-optical, label-free, and quantitative microscopy techniques, based on coherent Raman scattering, multiphoton absorption, and interferometry, to explore the early onset and progression of this phenotype, which has been understudied in unperturbed conditions. We identified TIS manifestations as early as 24 hours following treatment, consisting of substantial mitochondrial rearrangement and increase of volume and dry mass, followed by accumulation of lipid vesicles starting at 72 hours. This work holds the potential to affect anticancer treatment research, by offering a label-free, rapid, and accurate method to identify initial TIS in tumor cells.

논문정보

형식Research article
게재일2023년 09월 (BRIC 등록일 2023-09-15)
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