최수빈 (서울대학교) | 한빛사논문 > 한빛사

한빛사논문

조회398

최수빈Subin Choi

서울대학교

Adv. Healthc. Mater., Sep 07 2023 | https://doi.org/10.1002/adhm.202300998 Double Emulsion-Mediated Delivery of Polyphenol Mixture Alleviates Atopic Dermatitis.

Subin Choi^1†, Junghyeon Ko^2†, Su-Bin Park¹, Joo-YoungKim¹, Jung-Hwa Ha⁵, Sangho Roh⁶, Young-Hyeon An^2,3*, Nathaniel S. Hwang^1,2,3,4*

¹Interdisciplinary Program in Bioengineering, Seoul National University, Seoul, 08826, Republic of Korea.

²School of Chemical and Biological Engineering, Seoul National University, Seoul, 08826, Republic of Korea.

³Department of Social Welfare, Seoul National University, Seoul, 08826, Republic of Korea.

⁴Cellular Reprogramming and Embryo Biotechnology Laboratory, Dental Research Institute, Seoul National University School of Dentistry, Seoul, 08826, Republic of Korea.

⁵BioMax/N-Bio Institute, Seoul National University, Seoul, 08826, Republic of Korea.

⁶Institute of Engineering Research, Seoul National University, Seoul, 08826, Republic of Korea.

^*Corresponding authors: Young-Hyeon An, Nathaniel S. Hwang

^†These authors contributed equally to this work

Abstract

Although the polyphenols have been studied to alleviate inflammation, there are still challenges to delivering the polyphenols with stabilized formulation due to the low water solubility and susceptibility to oxidation. We herein developed the transdermal delivery system of polyphenol mixture (PM), including quercetin (Q), phloretin (P), and ellagic acid (E), using double emulsion for applying to atopic dermatitis (AD). Through the in vitro anti-degranulation assay, we obtained the optimal molar ratio of each polyphenol (Q:P:E = 5:1:1), and the PM showed at most a 43.6% reduction of degranulation of immune cells, which is the primary factor of AD. Moreover, the water-in-oil-in-water double emulsion (W/O/W) enhanced the PM's stability and had a higher anti-degranulation effect than the oil-in-water emulsion (O/W). In the in vivo 1-chloro-2,4-dinitrobenzene (DNCB)-induced mice AD model, PM reduced more AD symptoms than every single polyphenol. The PM-encapsulated W/O/W (PM_W/O/W) showed the most effectiveness in AD by decreasing dermatitis score, i.e., skin/ear thickness, mast cells, and serum IgE level. Finally, this suggests that our findings on the optimal ratio of PM and double emulsion-based delivery would be beneficial in treating AD and can be applied to other allergic diseases. This article is protected by copyright. All rights reserved.

논문정보

형식Research article
게재일2023년 09월 (BRIC 등록일 2023-09-18)
연구진국내 연구진
분야 바이오·의료융합 > 바이오소재

댓글 작성 로그인

CV 열람하기

연락처 보기