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Adv. Mater., Aug 25 2023 | https://doi.org/10.1002/adma.202302008
A 3D-Printed Assemblable Bespoke Scaffold as Versatile Microcryogel Carrier for Site-Specific Regenerative Medicine.
Seunghun S Lee 1, Nicole Kleger 2, Gisela A Kuhn 1, Helen Greutert 1, Xiaoyu Du 1, Thijs Smit 1, André R Studart 2, Stephen J Ferguson 1
1Institute for Biomechanics, Department of Health Sciences and Technology, ETH Zurich, Zurich, Switzerland.
2Complex Materials, Department of Materials, ETH Zurich, Zurich, Switzerland.
CORRESPONDING AUTHOR : Seunghun S. Lee
Abstract
Through the advent of additive manufacturing techniques, various approaches have been developed to fabricate patient-specific implant designs based on computed tomography (CT) images of the patient. However, this requires intensive work and can bring significant financial burden to the healthcare system. Here, we used a digital light processing to fabricate an hive-structured assemblable bespoke scaffold(HIVE). HIVE can be manually assembled in any shape/size with ease, so a surgeon can create a scaffold that would best fit a defect before implantation. Simultaneously, it can have site-specific treatments by working as a carrier filled with microcryogels(MC) incorporating different biological factors in different pockets of HIVE. After characterization, we investigated possible site-specific applications by utilizing HIVE as a versatile carrier with incorporated treatments such as growth factors(GF), bioceramic or cells. HIVE as a GF-carrier showed a controlled release of BMP-2/VEGF and induced osteogenesis/angiogenesis from human mesenchymal stem cells (hMSC) / human umbilical vein endothelial cells (HUVEC). Furthermore, as a bioceramic-carrier, HIVE demonstrated enhanced mineralization and osteogenesis, and as a HUVEC-carrier, it upregulated both osteogenic and angiogenic gene expression of hMSCs. A HIVE with different combinations of MCs yielded a distinct local effect and we confirmed successful cell migration within assembled HIVE scaffolds. Finally, an in vivo rat subcutaneous implantation study demonstrated site-specific osteogenesis and angiogenesis. This article is protected by copyright. All rights reserved.
논문정보
- Research article
- 2023년 08월 (BRIC 등록일 2023-09-18)
- 국외 연구진
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