한빛사논문
- 조회781
Linxia Liu 1,2,3,6, Jinlong Li 1,3,4,6, Yuanming Gai 1,2,6, Zhizhong Tian 1, Yanyan Wang 1, Tenghe Wang 1, Pi Liu 1, Qianqian Yuan 1, Hongwu Ma 1, Sang Yup Lee 5,* & Dawei Zhang 1,2,3,4,*
1Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China.
2National Technology Innovation Center of Synthetic Biology, Tianjin, China.
3Key Laboratory of Engineering Biology for Low-Carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China.
4University of Chinese Academy of Sciences, Beijing, China.
5Department of Chemical and Biomolecular Engineering (BK21 four program), Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea.
6These authors contributed equally: Linxia Liu, Jinlong Li, Yuanming Gai.
*Corresponding author: correspondence to Sang Yup Lee or Dawei Zhang
Abstract
Vitamin B6 is an essential nutrient with extensive applications in the medicine, food, animal feed, and cosmetics industries. Pyridoxine (PN), the most common commercial form of vitamin B6, is currently chemically synthesized using expensive and toxic chemicals. However, the low catalytic efficiencies of natural enzymes and the tight regulation of the metabolic pathway have hindered PN production by the microbial fermentation process. Here, we report an engineered Escherichia coli strain for PN production. Parallel pathway engineering is performed to decouple PN production and cell growth. Further, protein engineering is rationally designed including the inefficient enzymes PdxA, PdxJ, and the initial enzymes Epd and Dxs. By the iterative multimodule optimization strategy, the final strain produces 1.4 g/L of PN with productivity of 29.16 mg/L/h by fed-batch fermentation. The strategies reported here will be useful for developing microbial strains for the production of vitamins and other bioproducts having inherently low metabolic fluxes.
논문정보
- Research article
- 2023년 08월 (BRIC 등록일 2023-09-06)
- 국내(교신)+국외 연구진
- 생명과학 > 응용생물화학
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