한빛사논문
- 조회330
Hongwoo Lee1 and Pil Joon Seo 1,2,*
1Department of Chemistry, Seoul National University, Seoul 08826, Korea and
2Plant Genomics and Breeding Institute, Seoul National University, Seoul 08826, Korea
*To whom correspondence should be addressed.
Abstract
Three-dimensional (3D) chromatin structure is linked to transcriptional regulation in multicellular eukaryotes including plants. Taking advantage of high-resolution Hi-C (high-throughput chromatin conformation capture), we detected a small structural unit with 3D chromatin architecture in the Arabidopsis genome, which lacks topologically associating domains, and also in the genomes of tomato, maize, and Marchantia polymorpha. The 3D folding domain unit was usually established around an individual gene and was dependent on chromatin accessibility at the transcription start site (TSS) and transcription end site (TES). We also observed larger contact domains containing two or more neighboring genes, which were dependent on accessible border regions. Binding of transcription factors to accessible TSS/TES regions formed these gene domains. We successfully simulated these Hi-C contact maps via computational modeling using chromatin accessibility as input. Our results demonstrate that gene domains establish basic 3D chromatin architecture units that likely contribute to higher-order 3D genome folding in plants.
논문정보
- Research article
- 2023년 09월 (BRIC 등록일 2023-09-05)
- 국내 연구진
- 생명과학 > 식물학
관련 보도자료
- Bio통신원
- 2023. 09. 05
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