손기훈 (서울대학교)

한빛사논문

조회467

손기훈Kee Hoon Sohn

서울대학교

Nat. Genet., Aug 28 2023 | https://doi.org/10.1038/s41588-023-01486-9 Solanum americanum genome-assisted discovery of immune receptors that detect potato late blight pathogen effectors.

Xiao Lin^1,2,12 , Yuxin Jia^3,4,12, Robert Heal¹, Maxim Prokchorchik^5,11, Maria Sindalovskaya¹, Andrea Olave-Achury ¹, Moffat Makechemu1, Sebastian Fairhead¹, Azka Noureen¹, Jung Heo⁶, Kamil Witek¹, Matthew Smoker¹, Jodie Taylor¹, Ram-Krishna Shrestha 1, Yoonyoung Lee⁵, Chunzhi Zhang³, Soon Ju Park ^6,7, Kee Hoon Sohn^5,8,9,10 , Sanwen Huang³ & Jonathan D. G. Jones ¹

¹The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, Norwich, UK.

²State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.

³Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Area, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China.

⁴Key Laboratory for Potato Biology of Yunnan Province, The CAAS-YNNU-YINMORE Joint Academy of Potato Science, Yunnan Normal University, Kunming, China.

⁵Department of Life Sciences, Pohang University of Science and Technology, Pohang, Republic of Korea.

⁶Department of Biological Science and Institute of Basic Science, Wonkwang University, Iksan, Republic of Korea.

⁷Division of Applied Life Sciences and Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Gyeongsang National University, Jinju, Republic of Korea.

⁸School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, Pohang, Republic of Korea.

⁹Department of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea.

¹⁰Plant Immunity Research Center, Seoul National University, Seoul, Republic of Korea.

¹¹Present address: Plant Pathology Group, The Institute of Crop Science and Resource Conservation, University of Bonn, Bonn, Germany.

¹²These authors contributed equally: Xiao Lin, Yuxin Jia.

Corresponding authors : Correspondence to Xiao Lin, Kee Hoon Sohn, Sanwen Huang or Jonathan D. G. Jones.

Abstract

Potato (Solanum tuberosum) and tomato (Solanum lycopersicon) crops suffer severe losses to late blight caused by the oomycete pathogen Phytophthora infestans. Solanum americanum, a relative of potato and tomato, is globally distributed and most accessions are highly blight resistant. We generated high-quality reference genomes of four S. americanum accessions, resequenced 52 accessions, and defined a pan-NLRome of S. americanum immune receptor genes. We further screened for variation in recognition of 315P. infestans RXLR effectors in 52 S. americanum accessions. Using these genomic and phenotypic data, we cloned three NLR-encoding genes, Rpi-amr4, R02860 and R04373, that recognize cognate P. infestans RXLR effectors PITG_22825 (AVRamr4), PITG_02860 and PITG_04373. These genomic resources and methodologies will support efforts to engineer potatoes with durable late blight resistance and can be applied to diseases of other crops.

논문정보

형식Research article
게재일2023년 08월 (BRIC 등록일 2023-09-05)
연구진국내(교신)+국외 연구진
분야 생명과학 > 응용생물화학

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