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연세대학교

Biosens. Bioelectron., Oct 15 2023, 238 S0956-5663(23)00540-7 | https://doi.org/10.1016/j.bios.2023.115598 Phage-targeting bimetallic nanoplasmonic biochip functionalized with bacterial outer membranes as a biorecognition element.

Moon-Ju Kim a, Hyung Eun Bae a, Soonil Kwon a, Mi-Kyung Park b, Dongeun Yong c, Min-Jung Kang d, Jae-Chul Pyun a

aDepartment of Materials and Science and Engineering, Yonsei University, Seoul, 03722, South Korea

bSchool of Food Science and Biotechnology, Kyungpook National University, Daegu, 41566, South Korea

cDepartment of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, 03722, South Korea

dMolecular Recognition Research Center, Korea Institute of Science and Technology, Seoul, 02792, South Korea

Corresponding author : Jae-Chul Pyun

Abstract

The use of phages-a natural predator of bacteria-has emerged as a therapeutic strategy for treating multidrug-resistant bacterial infections; thus, the isolation and detection of phages from the environment is crucial for advancing phage therapy. Herein, for the first time, we propose a nanoplasmonic-based biodetection platform for phages that utilizes bacterial outer membranes (OMs) as a biorecognition element. Conventional biosensors based on phage-bacteria interactions encounter multiple challenges due to the bacteriolytic phages and potentially toxic bacteria, resulting in instability and risk in the measurement. Therefore, instead of whole living bacteria, we employ a safe biochemical OMs fraction presenting phage-specific receptors, allowing the robust and reliable phage detection. In addition, the biochip is constructed on bimetallic nanoplasmonic islands through solid-state dewetting for synergy between Au and Ag, whereby sensitive detection of phage-OMs interactions is achieved by monitoring the absorption peak shift. For high detection performance, the nanoplasmonic chip is optimized by systematically investigating the morphological features, e.g., size and packing density of the nanoislands. Using our optimized device, phages are detected with high sensitivity (≥∼10 ⁴ plaques), specificity (little cross-reactivity), and affinity (stronger binding to the host OMs than anti-bacterial antibodies), further exhibiting the cell-killing activities.

논문정보

형식Research article
게재일2023년 10월 (BRIC 등록일 2023-10-13)
연구진국내 연구진
분야 바이오·의료융합 > 바이오·의료융합 기타

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