Shujin Li (연세대학교)

한빛사논문

조회422

Shujin LiShujin Li

연세대학교

Chem. Eng. J., Available online 6 August 2023 | https://doi.org/10.1016/j.cej.2023.145211 Lipid anchor-mediated NK cell surface engineering for enhanced cancer immunotherapy

Sungjun Kim ^a,1, Shujin Li ^b,1, Mani Gajendiran ^a, Ashok Kumar Jangid ^a, Dong-Joon Lee ^b, Han-Sung Jung ^b, Kyobum Kim^a

^aDepartment of Chemical & Biochemical Engineering, Dongguk University, 30, Pildong-ro 1-gil, Jung-gu, Seoul, Republic of Korea
^bDivision in Anatomy and Developmental Biology, Department of Oral Biology, Taste Research Center, Oral Science Research Center, BK21 FOUR Project, Yonsei University College of Dentistry, 50-1 Yonsei-ro, Seodaemun-gu, Seoul, Republic of Korea

¹These authors contributed equally to this work.
Corresponding authors : Han-Sung Jung, Kyobum Kim

Abstract

Current natural killer (NK) cell-based cancer immunotherapy for the treatment of solid tumors often exhibits insufficient cancer recognition specificity, thereby limiting therapeutic anticancer efficacy, especially for triple-negative breast cancers (TNBCs). In this study, we develop artificial lipid-folate conjugates, for stable anchoring onto NK cell surfaces via hydrophobic interactions, thus augmenting folate-mediated ligand-receptor immune interactions with target cancers. This hydrophobized conjugate anchor provides additional cancer recognition ligands without any sophisticated genetic modification, and successfully enhances the anticancer efficacies of surface-coated NK (SCNK) cells without disturbing their intrinsic properties. Augmented cancer recognition ability sequentially promotes the secretion of cytolytic granules (granzyme and perforin) with cytokine (TNF-α), demonstrating improved cytotoxicity of the SCNK cells. Furthermore, the SCNK cells significantly infiltrate into the tumor site, inducing tumor apoptosis/necrosis, and suppressing tumor progression and metastasis in TNBC mouse models. Taken together, our artificial lipid-folate conjugates enable the treatment of solid tumors by augmenting the cancer-recognition and tumor targeting capacity of surface-engineered NK cells.

논문정보

형식Research article
게재일2023년 08월 (BRIC 등록일 2023-08-28)
연구진국내 연구진
분야 생명과학 > 생물공학

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