한빛사논문
- 조회908
Woochan Lee1,14, Seyoon Lee1,14, Jung-Ki Yoon2,14, Dakyung Lee1, Yuri Kim3, Yeon Bi Han4, Rokhyun Kim1, Sungji Moon5, Young Jun Park6, Kyunghyuk Park7, Bukyoung Cha7, Jaeyong Choi1, Juhyun Kim1, Na-young Ha3, Kwhanmien Kim8, Sukki Cho8, Nam-Hyuk Cho1,3,9, Tushar J. Desai2, Jin-Haeng Chung4,*, Joo-Hyeon Lee10,11,* and Jong-Il Kim1,5,12,13,*
1Department of Biomedical Sciences, Seoul National University College of Medicine, Seoul, Korea.
2Division of Pulmonary, Allergy and Critical Care, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA.
3Institute of Endemic Diseases, Medical Research Center, Seoul National University, Seoul, Korea.
4Department of Pathology and Translational Medicine, Seoul National University Bundang Hospital, Seongnam, Korea.
5Interdisciplinary Program in Cancer Biology, College of Medicine, Seoul National University, Seoul, Korea.
6Department of Translational Medicine, Seoul National University College of Medicine, Seoul, Korea.
7Genomic Medicine Institute (GMI), Medical Research Center, Seoul National University, Seoul, Korea.
8Department of Thoracic and Cardiovascular Surgery, Seoul National University Bundang Hospital, Seongnam, Korea.
9Department of Microbiology and Immunology, Seoul National University College of Medicine, Seoul, Korea.
10Wellcome–MRC Cambridge Stem Cell Institute, Jeffrey Cheah Biomedical Centre, University of Cambridge, Cambridge, UK.
11Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, UK.
12Department of Biochemistry and Molecular Biology, Seoul National University College of Medicine, Seoul, Korea.
13Cancer Research Institute, Seoul National University, Seoul, Korea.
14These authors contributed equally: Woochan Lee, Seyoon Lee, Jung-Ki Yoon.
*Corresponding author: correspondence to Jin-Haeng Chung, Joo-Hyeon Lee or Jong-Il Kim
Abstract
We present an in-depth single-cell atlas of in vitro multiculture systems on human primary airway epithelium derived from normal and diseased lungs of 27 individual donors. Our large-scale single-cell profiling identified new cell states and differentiation trajectories of rare airway epithelial cell types in human distal lungs. By integrating single-cell datasets of human lung tissues, we discovered immune-primed subsets enriched in lungs and organoids derived from patients with chronic respiratory disease. To demonstrate the full potential of our platform, we further illustrate transcriptomic responses to various respiratory virus infections in vitro airway models. Our work constitutes a single-cell roadmap for the cellular and molecular characteristics of human primary lung cells in vitro and their relevance to human tissues in vivo.
논문정보
- Research article
- 2023년 08월 (BRIC 등록일 2023-08-17)
- 국내(교신)+국외 연구진
- 의약학 > 유전 및 유전체의학
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