한빛사논문
- 조회577
Pratyush Kumar Mishra1*, Nirmali Sharma1, Hyunwoo Kim2, Changwook Lee2, and Hyun-Woo Rhee1,3*
1Department of Chemistry, Seoul National University, Seoul 08826, Republic of Korea
2Department of Biological Sciences, Ulsan National Institute of Science and Technology, Ulsan 44919, Republic of Korea
3School of Biological Sciences, Seoul National University, Seoul 08826, Republic of Korea
Corresponding Authors : Pratyush Kumar Mishra, Hyun-Woo Rhee
Abstract
Chemical reactions for the in situ modification of biomolecules within living cells are under development. Among these reactions, bio-orthogonal reactions such as click chemistry using copper(I) and Staudinger ligation are widely used for specific biomolecule tracking in live systems. However, currently available live cell copper(I)-catalyzed azide/alkyne cycloaddition reactions are not designed in a spatially resolved manner. Therefore, we developed the “GEN-Click” system, which can target the copper(I)-catalyzed azide/alkyne cycloaddition reaction catalysts proximal to the protein of interest and can be genetically expressed in a live cell. The genetically controlled, spatially restricted, metal-catalyzed biorthogonal reaction can be used for proximity biotin labeling of various azido-bearing biomolecules (e.g., protein, phospholipid, oligosaccharides) in living cell systems. Using GEN-Click, we successfully detected local metabolite-transferring events at cell–cell contact sites.
논문정보
- Research article
- 2023년 07월 (BRIC 등록일 2023-08-16)
- 국내 연구진
- 생명과학 > 세포생물학
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