고대관 (Michigan State University) | 한빛사논문 > 한빛사

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조회694

Michigan State University

Nat. Plants, Published: 10 August 2023 | https://doi.org/10.1038/s41477-023-01480-3 An IRE1-proteasome system signalling cohort controls cell fate determination in unresolved proteotoxic stress of the plant endoplasmic reticulum

Dae Kwan Ko^1,2, Joo Yong Kim¹, Ethan A. Thibault^1,3 & Federica Brandizzi^1,2,3

¹MSU-DOE Plant Research Lab, Michigan State University, East Lansing, MI, USA
²Great Lakes Bioenergy Research Center, Michigan State University, East Lansing, MI, USA
³Department of Plant Biology, Michigan State University, East Lansing, MI, USA

Corresponding author : Correspondence to Federica Brandizzi.

Abstract

Excessive accumulation of misfolded proteins in the endoplasmic reticulum (ER) causes ER stress, which is an underlying cause of major crop losses and devastating human conditions. ER proteostasis surveillance is mediated by the conserved master regulator of the unfolded protein response (UPR), Inositol Requiring Enzyme 1 (IRE1), which determines cell fate by controlling pro-life and pro-death outcomes through as yet largely unknown mechanisms. Here we report that Arabidopsis IRE1 determines cell fate in ER stress by balancing the ubiquitin–proteasome system (UPS) and UPR through the plant-unique E3 ligase, PHOSPHATASE TYPE 2CA (PP2CA)-INTERACTING RING FINGER PROTEIN 1 (PIR1). Indeed, PIR1 loss leads to suppression of pro-death UPS and the lethal phenotype of an IRE1 loss-of-function mutant in unresolved ER stress in addition to activating pro-survival UPR. Specifically, in ER stress, PIR1 loss stabilizes ABI5, a basic leucine zipper (bZIP) transcription factor, that directly activates expression of the critical UPR regulator gene, bZIP60, triggering transcriptional cascades enhancing pro-survival UPR. Collectively, our results identify new cell fate effectors in plant ER stress by showing that IRE1’s coordination of cell death and survival hinges on PIR1, a key pro-death component of the UPS, which controls ABI5, a pro-survival transcriptional activator of bZIP60.

논문정보

형식Research article
게재일2023년 08월 (BRIC 등록일 2023-08-11)
연구진국외 연구진
분야 생명과학 > 식물학

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