한빛사논문
- 조회737
Eun Song Lee1,†, Jung Heo1,2,†, Woo Young Bang3,†, Kapeel M. Chougule4,†, Nomar Espinosa Waminal5,6,Nguyen Thi Hong6, Min Ji Kim1, Hong Kwan Beak1, Yong Jun Kim1, Ryza A. Priatama1,7, Ji In Jang1,2, Kang Il Cha1, Seung Han Son1, Sujeevan Rajendran1, Young-Kug Choo1, Jong Hyang Bae8, Chul Min Kim8, Young Koung Lee7, Sangsu Bae9, Jonathan D. G. Jones10, Kee Hoon Sohn11, Jiyoung Lee12, Hyun Hee Kim6, Jong Chan Hong2, Doreen Ware4,13,*, Keunhwa Kim1,2,* and Soon Ju Park1,2,*
1Division of Biological Sciences, Wonkwang University, Iksan, Korea
2Division of Applied Life Science (BK21 four) and Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Gyeongsang National University, Jinju, Korea
3Biological and Genetic Resources Assessment Division, National Institute of Biological Resources, Incheon, Korea
4Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA
5Leibniz Institute of Plant Genetics and Crop Plant Research, Gatersleben, Germany
6BioScience Institute, Department of Chemistry &Life Science, Sahmyook University, Seoul, Korea
7Institute of Plasma Technology, Korea Institute of Fusion Energy, Gunsan-si, Korea
8Division of Horticulture Industry, Wonkwang University, Iksan, Korea
9Department of Biomedical Sciences, Seoul National University College of Medicine, Seoul, South Korea
10The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, Norwich, UK
11Department of Agricultural Biotechnology, Plant Immunity Research Center, Research Institute of Agriculture and Life Sciences, Seoul National University,Seoul, Korea
12Korean Collection for Type Cultures (KCTC), Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup, Korea
13U.S. Department of Agriculture-Agricultural Research Service, NEA Robert W. Holley Center for Agriculture and Health, Ithaca, NY, USA
†These authors contributed equally to this work
*Corresponding author: correspondence to Doreen Ware, Keunhwa Kim or Soon Ju Park
Abstract
Numerous staple crops exhibit polyploidy and are difficult to genetically modify. However, recent advances in genome sequencing and editing have enabled polyploid genome engineering. The hexaploid black nightshade species Solanum nigrum has immense potential as a beneficial food supplement. We assembled its genome at the scaffold level. After functional annotations, we identified homoeologous gene sets, with similar sequence and expression profiles, based on comparative analyses of orthologous genes with close diploid relatives Solanum americanum and S. lycopersicum. Using CRISPR-Cas9-mediated mutagenesis, we generated various mutation combinations in homoeologous genes. Multiple mutants showed quantitative phenotypic changes based on the genotype, resulting in a broad-spectrum effect on the quantitative traits of hexaploid S. nigrum. Furthermore, we successfully improved the fruit productivity of Boranong, an orphan cultivar of S. nigrum suggesting that engineering homoeologous genes could be useful for agricultural improvement of polyploid crops.
논문정보
- Research article
- 2023년 08월 (BRIC 등록일 2023-08-04)
- 국내(교신)+국외 연구진
- 생명과학 > 생리학
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