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성균관대학교 의과대학 정밀의학교실

Chem. Eng. J., Available online 23 July 2023 | https://doi.org/10.1016/j.cej.2023.144979 Formation of various cell-aggregated structures in the core of hydrogel filament using a microfluidic device and its application as an in vitro neuromuscular junction model

JuYeon Kim^a, GeunHyung Kim ^a,b,c

^aDepartment of Precision Medicine, Sungkyunkwan University School of Medicine (SKKU-SOM), Suwon 16419, Republic of Korea
^bInstitute of Quantum Biophysics, Department of Biophysics, Sungkyunkwan University, Suwon, Gyeonggi-do 16419, Republic of Korea
^cBiomedical Institute for Convergence at SKKU (BICS), Sungkyunkwan University, Suwon 16419, Republic of Korea

Corresponding author : GeunHyung Kim

Abstract

Microfluidic systems have been widely used in various in vitro tissue engineering models. In this paper, we present a new microfluidic system for manufacturing cell filaments laden with cell aggregates of various shapes (beads, rosaries, and filaments) in the core region. The structures were obtained using the Rayleigh-Plateau instability of the two-phase flow of the injected bioinks (continuous phase of collagen/poly[ethylene glycol] diacrylate [PEGDA] and a dispersed phase of methacrylated gelatin (GelMA)). To demonstrate the potential of the system at the neuromuscular junction in vitro model, we injected a C2C12-laden collagen/PEGDA bioink into the sheath region and GelMA mixed with motor neuron cells (NSC-34) into the core region of the fabricated cell aggregates. The rosary-shaped NSC-34 aggregate in the core exhibited higher cell viability and more developed neurogenic gene expression than the cell aggregates of beads and filaments in the core region, owing to much greater mechanotransduction. Furthermore, when comparing the cell-laden struts (C2C12 in the sheath region and NSC34 in the rosary core region) with normally bioprinted struts with the same cell density, the expressions of myogenesis-, neuromuscular junction (NMJ)-, and neurogenesis-related genes in struts laden with rosary-shaped cell aggregates were significantly upregulated. The results of these experiments demonstrate the potential for the fabrication of new cell-laden core-sheath structures, which could be used for designing various in vitro models.

논문정보

형식Research article
게재일2023년 07월 (BRIC 등록일 2023-08-10)
연구진국내 연구진
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