한빛사논문
Gyu Tae Park1, Jae Kyung Lim1, Eun-Bae Choi1, Mi-Ju Lim1, Bo-Young Yun2, Dae Kyoung Kim1, Jung Won Yoon1, Yoon Gi Hong3, Jae Hoon Chang3, Seong Hwan Bae4, Jung Yong Ahn2* and Jae Ho Kim1,5*
1Department of Physiology, College of Medicine, Pusan National University, Yangsan, Gyeongsangnam-do 50612, Republic of Korea
2UVA Surgery Clinic, Busan 47537, Republic of Korea
3BS The Body Aesthetic Plastic Surgery Clinic, Busan 47287, Republic of Korea
4Department of Plastic and Reconstructive Surgery, College of Medicine, Pusan National University, Busan, Gyeongsangnam-do 49241, Republic of Korea
5Department of Physiology, Pusan National University School of Medicine, Yangsan, Gyeongsangnam-do 50612, Republic of Korea
*Correspondence: Jung Yong Ahn, Jae Ho Kim
Abstract
Background: Adipose tissue-derived microvascular fragments are functional vessel segments derived from arterioles, capillaries, and veins. Microvascular fragments can be used as vascularization units in regenerative medicine and tissue engineering containing microvascular networks. However, the in vivo therapeutic and vascularization properties of human microvascular fragments have not been investigated.
Methods: In this study, we isolated microvascular fragments, stromal vascular fractions, and mesenchymal stem cells from human lipoaspirate and studied their therapeutic efficacy and in vivo vasculogenic activity in a murine model of hindlimb ischemia. In addition, in vivo angiogenic activity and engraftment of microvascular fragments into blood vessels were measured using Matrigel plug assay.
Results: Both microvascular fragments and stromal vascular fractions contain not only mesenchymal stem cells but also endothelial progenitor cells. In a Matrigel plug assay, microvascular fragments increased the number of blood vessels containing red blood cells more than mesenchymal stem cells and stromal vascular fractions did. The engraftment of the microvascular fragments transplanted in blood vessels within the Matrigel plug significantly increased compared to the engraftment of mesenchymal stem cells and stromal vascular fractions. Moreover, intramuscular injection of microvascular fragments markedly increased blood flow in the ischemic hindlimbs and alleviated tissue necrosis compared to that of mesenchymal stem cells or stromal vascular fractions. Furthermore, transplanted microvascular fragments formed new blood vessels in ischemic limbs.
Conclusions: These results suggest that microvascular fragments show improved engraftment efficiency and vasculogenic activity in vivo and are highly useful for treating ischemic diseases and in tissue engineering. Adipose tissue-derived microvascular fragments are vascularization units in regenerative medicine and tissue engineering containing microvascular networks. Intramuscular injection of microvascular fragments markedly increased blood flow in the ischemic hindlimbs and alleviated tissue necrosis. The present study suggests that microvascular fragments show improved engraftment efficiency and vasculogenic activity in vivo and are highly useful for treating ischemic diseases and in tissue engineering.
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