한빛사논문
- 조회363
Shujie Yang1, Joseph Rufo1, Ruoyu Zhong1, Joseph Rich 2, Zeyu Wang1, Luke P. Lee3,4,5 & Tony Jun Huang1
1Thomas Lord Department of Mechanical Engineering and Materials Science, Duke University, Durham, NC, USA.
2Department of Biomedical Engineering, Duke University, Durham, NC, USA.
3Renal Division and Division of Engineering in Medicine, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, USA.
4Department of Bioengineering, Department of Electrical Engineering and Computer Science, University of California, Berkeley, Berkeley, CA, USA.
5Institute of Quantum Biophysics, Department of Biophysics, Sungkyunkwan University, Suwon, South Korea.
Corresponding authors : Correspondence to Luke P. Lee or Tony Jun Huang.
Abstract
Acoustic tweezers provide an effective means for manipulating single cells and particles in a high-throughput, precise, selective and contact-free manner. The adoption of acoustic tweezers in next-generation cellular assays may advance our understanding of biological systems. Here we present a comprehensive set of instructions that guide users through device fabrication, instrumentation setup and data acquisition to study single cells with an experimental throughput that surpasses traditional methods, such as atomic force microscopy and micropipette aspiration, by several orders of magnitude. With acoustic tweezers, users can conduct versatile experiments that require the trapping, patterning, pairing and separation of single cells in a myriad of applications ranging across the biological and biomedical sciences. This procedure is widely generalizable and adaptable for investigations in materials and physical sciences, such as the spinning motion of colloids or the development of acoustic-based quantum simulations. Overall, the device fabrication requires ~12 h, the experimental setup of the acoustic tweezers requires 1-2 h and the cell manipulation experiment requires ~30 min to complete. Our protocol is suitable for use by interdisciplinary researchers in biology, medicine, engineering and physics.
논문정보
- Review Paper
- 2023년 07월 (BRIC 등록일 2023-08-03)
- 국내(교신)+국외 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
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