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조회1,147

Texas A&M University, 공주대학교

Mol. Plant., 2023 Jul 11;S1674-2052(23)00179-X | https://doi.org/10.1016/j.molp.2023.07.003 9,10-KODA, an α-ketol produced by the tonoplast-localized 9-lipoxygenase ZmLOX5, plays a signaling role in maize defense against insect herbivory

Peiguo Yuan ^1,11, Eli Borrego ^1,2,11, Yong-Soon Park ^1,10,11, Zachary Gorman¹, Pei-Cheng Huang ¹, Jordan Tolley³, Shawn A. Christensen ^1,4, Jantana Blanford ⁵, Aruna Kilaru ⁶, Robert Meeley ⁷, Hisashi Koiwa ³, Stefan Vidal ⁸, Alisa Huffaker ⁹, Eric Schmelz ⁹, Michael V. Kolomiets ¹

¹Department of Plant Pathology and Microbiology, Texas A&M University, College Station, TX 77840-2132, USA

²Currently at Thomas H. Gosnell School of Life Sciences, Rochester Institute of Technology, Rochester, NY 14623, USA

³Department of Horticultural Sciences, Texas A&M University, College Station, TX 77843, USA

⁴College of Life Sciences, Brigham Young University, Provo, UT 84602, USA

⁵Biology Department, Brookhaven National Laboratory, Upton, NY 11973, USA

⁶Department of Biological Sciences, East Tennessee State University, Johnson City, TN 37659, USA

⁷Formerly at Corteva Agriscience, Johnston, IA 50131, USA

⁸Department of Crop Sciences, Agricultural Entomology, Georg-August-Universität, 37077 Göttingen, Germany

⁹Section of Cell and Developmental Biology, University of California at San Diego, La Jolla, CA 92037, USA

¹⁰Department of Plant Resources, Agriculture and Fisheries Life Science Research Institute, Kongju National University, Yesan, Chungnam 32439, South Korea

¹¹These authors contributed equally to this article.

Corresponding author : Michael V. Kolomiets

Abstract

13-lipoxygenases (LOXs) initiate the synthesis of jasmonic acid (JA), the best-understood oxylipin hormone in herbivory defense. However, the roles of 9-LOX-derived oxylipins in insect resistance remain unclear. Here, we report a novel anti-herbivory mechanism mediated by a tonoplast-localized 9-LOX, ZmLOX5, and its linolenic acid-derived product, 9-hydroxy-10-oxo-12(Z),15(Z)-octadecadienoic acid (9,10-KODA). Transposon-insertional disruption of ZmLOX5 resulted in the loss of resistance to insect herbivory. lox5 knock-out mutants displayed greatly reduced wound-induced accumulation of multiple oxylipins and defense metabolites, including benzoxazinoids, abscisic acid (ABA) and JA-isoleucine (JA-Ile). However, exogenous JA-Ile failed to rescue insect defense in lox5 mutants, while applications of 1 μM 9,10-KODA or the JA precursor, 12-oxo-phytodienoic acid (12-OPDA), restored wild-type resistance levels. Metabolite profiling revealed that exogenous 9,10-KODA primed the plants for increased production of ABA and 12-OPDA, but not JA-Ile. While none of the 9-oxylipins were able to rescue JA-Ile induction, the lox5 mutant accumulated lower wound-induced levels of Ca2+,suggesting this as a potential explanation for lower wound-induced JA. Seedlings pretreated with 9,10-KODA exhibited rapid or more robust wound-induced defense gene expression. In addition, an artificial diet supplemented with 9,10-KODA arrested fall armyworm larvae growth. Finally, analysis of single and double lox5 and lox10 mutants showed that ZmLOX5 also contributed to insect defense by modulating ZmLOX10-mediated green leaf volatile signaling. Our study uncovered a previously unknown antiherbivore defense and hormone-like signaling activity for a major 9-oxylipin α-ketol.

논문정보

형식Research article
게재일2023년 07월 (BRIC 등록일 2023-07-28)
연구진국외 연구진
분야 생명과학 > 식량작물 및 원예작물

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