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ACS Appl. Mater. Interfaces, Publication Date:June 29, 2023 | https://doi.org/10.1021/acsami.3c06086 Synthetic Receptor Based on a Peptide Antibiotic-Functionalized Chimera for Hybridization-Based Polynucleotide Detection

Loredana Mereuta ¹, Alina Asandei ², Irina Schiopu ², Jonggwan Park ³, Yoonkyung Park ⁴, Tudor Luchian¹

¹Department of Physics, Alexandru I. Cuza University, 700506 Iasi, Romania.
²Interdisciplinary Research Institute, Sciences Department, Alexandru I. Cuza University, 700506 Iasi, Romania.
³Department of Bioinformatics, Kongju National University, Kongju 32588, Republic of Korea.
⁴Department of Biomedical Science and Research Center for Proteinaceous Materials (RCPM), Chosun University, Gwangju 61452, Republic of Korea.

Corresponding Authors : Yoonkyung Park, Tudor Luchian

Abstract

Nanopores offer highly sensitive, low-cost, and single-molecule sensing capabilities, and the societal impact of this approach is best captured by the advent of nanopore-based DNA detection and sequencing technologies, which extract genomic information without amplification. To address a critical difficulty plaguing such undertakings involving especially protein-based nanopores isolated in lipid bilayers, namely, the formation of a stable, long-lasting single nanopore, we pioneer herein an approach for generating functional nanostructures enabling small single-stranded DNA (ssDNA) detection. We designed a dynamic hybrid construct by appending extramembrane peptide nucleic acid (PNA) segments to the C-terminus of modified ion channel-forming alamethicin monomers. We found that the resulting chimeric molecules successfully coassemble in a voltage-dependent manner in planar lipid membranes generating diameter-variable oligomers. The subsequent interaction at the flexible extramembrane segment of such formed dynamic nanopores with aqueously added complementary ssDNA fragments leads to overall conformational alterations affecting the peptide assembly state kinetics and mediated ionic current. Such recognition events were found specific to the primary structure of target ssDNA and uninhibited the presence of serum. Our platform demonstrates the feasibility of designing an entirely new class of versatile chimeric biosensors, for which, dependent upon the nature of the attached receptor moiety and underlying recognition chemistry, the applicability area may extend to other analytes.

논문정보

형식Research article
게재일2023년 06월 (BRIC 등록일 2023-07-23)
연구진국내(교신)+국외 연구진
분야 생명과학 > 면역학

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