한빛사논문
Minseong Kim 1, Mamata S. Singhvi 1, Beom Soo Kim
Department of Chemical Engineering, Chungbuk National University, Cheongju, Chungbuk 28644, Republic of Korea
1Both authors contributed equally in this work.
Corresponding author : Beom Soo Kim
Abstract
Cerium-doped iron oxide nanoparticles (CeFe3O4-NPs) possessing exceptional laccase and cellulase/hemicellulase mimicking properties were exploited for simultaneous pretreatment (i.e. delignification) and saccharification (SPS) of raw corn cob (CC) biomass. Here, we developed an eco-friendly single step SPS strategy for conversion of raw CC biomass into C6 and C5 sugars using CeFe3O4-NPs with in-house produced cellulase and hemicellulase enzymes which generated maximum glucose (20.3 ± 1.01 g/L) and xylose (22.0 ± 2.22 g/L) within 24 h of hydrolysis at 55 °C and further 21.7 g/L of bioethanol was produced by Saccharomyces cerevisiae. These results clearly elucidated the role of CeFe3O4-NPs as lignocellulose degrading laccase and cellulase/hemicellulase enzymes. Remarkably, the presence of CeFe3O4-NPs has triggered xylose utilization in S. cerevisiae which is completely a new finding. This study can provide new insights towards the probable role of CeFe3O4-NPs in the up-regulation of significant genes responsible for xylose utilization which is a noteworthy finding in the field of ‘biomass to biofuel’ conversion.
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