곽태준 (Harvard Medical School / Massachusetts General Hospital) | 한빛사논문 > 한빛사

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조회496

Harvard Medical School / Massachusetts General Hospital

CV File 221 kb

Biosens. Bioelectron., 2023 Jun 3;237:115422 | https://doi.org/10.1016/j.bios.2023.115422 Electrokinetically enhanced label-free plasmonic sensing for rapid detection of tumor-derived extracellular vesicles

Tae Joon Kwak ^a, Taehwang Son ^a, Jae-Sang Hong ^a, Ursula Andrea Winter ^a, Mi Ho Jeong ^a, Charlotte McLean ^a,b, Ralph Weissleder ^a,c,d,e, Hakho Lee^a,c, Cesar M. Castro^a,d, Hyungsoon Im^a,c

^aCenter for Systems Biology, Massachusetts General Hospital, Boston, MA, 02114, USA
^bDepartment of Health Science, Northeastern University Boston, MA, 02115, USA
^cDepartment of Radiology, Massachusetts General Hospital, Boston, MA, 02114, USA
^dCancer Center, Massachusetts General Hospital, Boston, MA, 02114, USA
^eDepartment of Systems Biology, Harvard Medical School, Boston, MA, 02115, USA

Corresponding author : Hyungsoon Im

Abstract

of rare circulating extracellular vesicles (EV) from early cancers or different types of host cells requires extremely sensitive EV sensing technologies. Nanoplasmonic EV sensing technologies have demonstrated good analytical performances, but their sensitivity is often limited by EVs' diffusion to the active sensor surface for specific target EV capture. Here, we developed an advanced plasmonic EV platform with electrokinetically enhanced yields (KeyPLEX). The KeyPLEX system effectively overcomes diffusion-limited reactions with applied electroosmosis and dielectrophoresis forces. These forces bring EVs toward the sensor surface and concentrate them in specific areas. Using the keyPLEX, we showed significant improvements in detection sensitivity by ∼100-fold, leading to the sensitive detection of rare cancer EVs from human plasma samples in 10 min. The keyPLEX system could become a valuable tool for point-of-care rapid EV analysis.

논문정보

형식Research article
게재일2023년 06월 (BRIC 등록일 2023-07-10)
연구진국외 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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