한빛사논문
- 조회289
Alfonsina Milito 1,†, Moritz Aschern 1,†, Josie L. McQuillan 2 and Jae-Seong Yang 1,*
1Centre for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UB, Campus UAB, Bellaterra, Barcelona, Spain
2Department of Chemical and Biological Engineering, University of Sheffield, Mappin Street, Sheffield, S1 3JD, UK
†These authors equally contributed to this work.
*Corresponding author: correspondence to Jae-Seong Yang
Abstract
Microalgae hold the enormous potential to provide a safe and sustainable source of high-value compounds, acting as carbon-fixing biofactories that could help to mitigate rapidly progressing climate change. Bioengineering microalgal strains will be key to optimizing and modifying their metabolic outputs, and to render them competitive with established industrial biotechnology hosts, such as bacteria or yeast. To achieve this, precise and tunable control over transgene expression will be essential, towards which a key strategy is the development and rational design of synthetic promoters. Among green microalgae, Chlamydomonas reinhardtii represents the reference species for bioengineering and synthetic biology; however, the repertoire of functional synthetic promoters for this species, and for microalgae generally, is limited in comparison to other commercial chassis, emphasizing the need to expand the current microalgal gene expression toolbox. Here, we discuss state-of-the-art promoter analyses and highlight areas of research required to advance synthetic promoter development in C. reinhardtii. In particular, we exemplify high-throughput studies performed in other model systems that could be applicable to microalgae and propose novel approaches to interrogating algal promoters. We lastly outline the major limitations hindering microalgal promoter development, while providing novel suggestions and perspectives for how to overcome them.
논문정보
- Review Paper
- 2023년 04월 (BRIC 등록일 2023-07-07)
- 국외 연구진
- 생명과학 > 유전자발현
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