한빛사논문
- 조회1,594
Jenny J. Seo,1,2,3 Soo-Jin Jung,1,2,3 Jihye Yang,1,2 Da-Eun Choi,1,2 and V. Narry Kim1,2,4,*
1Center for RNA Research, Institute for Basic Science, Seoul 08826, Republic of Korea
2School of Biological Sciences, Seoul National University, Seoul 08826, Republic of Korea
3These authors contributed equally
4Lead contact
*Corresponding author: correspondence to V. Narry Kim
Abstract
The number of sequenced viral genomes has surged recently, presenting an opportunity to understand viral diversity and uncover unknown regulatory mechanisms. Here, we conducted a screening of 30,367 viral segments from 143 species representing 96 genera and 37 families. Using a library of viral segments in 3′ UTR, we identified hundreds of elements impacting RNA abundance, translation, and nucleocytoplasmic distribution. To illustrate the power of this approach, we investigated K5, an element conserved in kobuviruses, and found its potent ability to enhance mRNA stability and translation in various contexts, including adeno-associated viral vectors and synthetic mRNAs. Moreover, we identified a previously uncharacterized protein, ZCCHC2, as a critical host factor for K5. ZCCHC2 recruits the terminal nucleotidyl transferase TENT4 to elongate poly(A) tails with mixed sequences, delaying deadenylation. This study provides a unique resource for virus and RNA research and highlights the potential of the virosphere for biological discoveries.
논문정보
- Research article
- 2023년 07월 (BRIC 등록일 2023-07-12)
- 국내 연구진
- 생명과학 > 세포생물학
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