한빛사논문
- 조회351
Ji-Sun Park 1,2, Kwang Hyun Park 3, Su-Jin Park 1,4, Seo-Rin Ko 1, Ki-Beom Moon 1, Hyunjin Koo 1, Hye Sun Cho 1,4, Sang Un Park 2, Jae-Heung Jeon 1, Hyun-Soon Kim 1,4, Hyo-Jun Lee 1,5,6
1Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, South Korea.
2Department of Crop Science, Chungnam National University, Daejeon 34134, South Korea.
3Disease Target Structure Research Center, Korea Research Institute of Bioscience & Biotechnology, Daejeon 34141, South Korea.
4Department of Biosystems and Bioengineering, KRIBB School of Biotechnology, University of Science and Technology, Daejeon 34113, South Korea.
5Department of Functional Genomics, KRIBB School of Bioscience, University of Science and Technology, Daejeon 34113, South Korea.
6Department of Biological Sciences, Sungkyunkwan University, Suwon 16419, Korea.
Author for correspondence: Hyun-Soon Kim, Hyun-Soon Kim
Abstract
Plant cells can reprogram their fate. The combinatorial actions of auxin and cytokinin dedifferentiate somatic cells to regenerate organs, which can develop into individual plants. As transgenic plants can be generated from genetically modified somatic cells through these processes, cell fate transition is an unavoidable step in crop genetic engineering. However, regeneration capacity closely depends on the genotype, and the molecular events underlying these variances remain elusive. In the present study, we demonstrated that WUSCHEL (WUS) -a homeodomain transcription factor-determines regeneration capacity in different potato (Solanum tuberosum) genotypes. Comparative analysis of shoot regeneration efficiency and expression of genes related to cell fate transition revealed that WUS expression coincided with regeneration rate in different potato genotypes. Moreover, in a high-efficiency genotype, WUS silencing suppressed shoot regeneration. Meanwhile, in a low-efficiency genotype, regeneration could be enhanced through the supplementation of a different type of cytokinin that promoted WUS expression. Computational modeling of cytokinin receptor-ligand interactions suggested that the docking pose of cytokinins mediated by hydrogen bonding with the core residues may be pivotal for WUS expression and shoot regeneration in potatoes. Furthermore, our whole genome sequencing analysis revealed core sequence variations in the WUS promoters that differentiate low- and high-efficiency genotypes. The present study revealed that cytokinin responses, particularly WUS expression, determine shoot regeneration efficiency in different potato genotypes.
논문정보
- Research article
- 2023년 06월 (BRIC 등록일 2023-07-05)
- 국내 연구진
- 생명과학 > 식물학
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