홍정주 (한국생명공학연구원, UST) | 한빛사논문 > 한빛사

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조회949

한국생명공학연구원, UST

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J. Med. Virol., 2023 Jun;95(6):e2884 | https://doi.org/10.1002/jmv.28847 Comparative spatial transcriptomic profiling of severe acute respiratory syndrome coronavirus 2 Delta and Omicron variants infections in the lungs of cynomolgus macaques

Taehwan Oh ¹, Green Kim ¹, Seung Ho Baek ¹, YoungMin Woo ^1,2, Bon-Sang Koo ¹, Eun-Ha Hwang ¹, Kyuyoung Shim ^1,2, You Jung An ¹, Yujin Kim ¹, Jae-Hak Park ³, Jung Joo Hong ^1,2

¹Korea Research Institute of Bioscience and Biotechnology (KRIBB), National Primate Research Centre, Cheongju, Chungcheongbuk, Republic of Korea.
²KRIBB School of Bioscience, Korea University of Science & Technology (UST), Daejeon, Republic of Korea.
³Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea.

Correspondence : Jung Joo Hong

Abstract

Recently emerging severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron variants are generally less pathogenic than previous strains. However, elucidating the molecular basis for pulmonary immune response alterations is challenging owing to the virus's heterogeneous distribution within complex tissue structure. Here, we revealed the spatial transcriptomic profiles of pulmonary microstructures at the SARS-CoV-2 infection site in the nine cynomolgus macaques upon inoculation with the Delta and Omicron variants. Delta- and Omicron-infected lungs had upregulation of genes involved in inflammation, cytokine response, complement, cell damage, proliferation, and differentiation pathways. Depending on the tissue microstructures (alveoli, bronchioles, and blood vessels), there were differences in the types of significantly upregulated genes in each pathway. Notably, a limited number of genes involved in cytokine and cell damage response were differentially expressed between bronchioles of the Delta- and Omicron-infection groups. These results indicated that despite a significant antigenic shift in SARS-CoV-2, the host immune response mechanisms induced by the variants were relatively consistent, with limited transcriptional alterations observed only in large airways. This study may aid in understanding the pathogenesis of SARS-CoV-2 and developing a clinical strategy for addressing immune dysregulation by identifying potential transcriptional biomarkers within pulmonary microstructures during infection with emerging variants.

논문정보

형식Research article
게재일2023년 06월 (BRIC 등록일 2023-07-13)
연구진국내 연구진
분야 의약학 > 감염의학

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