이상수 (연세대학교)

한빛사논문

조회246

연세대학교

J. Hazard. Mater., Available online 15 June 2023 | https://doi.org/10.1016/j.jhazmat.2023.131862 Pleiotropic melatonin-mediated responses on growth and cadmium phytoextraction of Brassica napus: A bioecological trial for enhancing phytoremediation of soil cadmium

Saiqa Menhas ^a,b,c,1, Xijia Yang ^a,b,1, Kashif Hayat^a,d, Jochen Bundschuh ^e,f, Xunfeng Chen ^a,b,c, Nan Hui ^a,b,c, Dan Zhang ^a,b,c, Shaohua Chu ^a,b,c, Yuanfei Zhou ^a,b,c, Esmat F. Ali ^g, Muhammad Shahid ^h, Jörg Rinklebe ⁱ, Sang Soo Lee ^j, Sabry M. Shaheen ^i,k,l, Pei Zhou ^a,b,c

^aSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, PR China
^bKey Laboratory of Urban Agriculture, Ministry of Agriculture and Rural Affairs, Shanghai, PR China
^cShanghai Yangtze River Delta Eco-Environmental Change and Management Observation and Research Station, Ministry of Science and Technology, Ministry of Education, Shanghai 200240, PR China
^dKey Laboratory of Pollution Exposure and Health Intervention, Interdisciplinary Research Academy, Zhejiang Shuren University, Hangzhou 310015, PR China
^eDepartment of Earth and Environmental Sciences, National Chung Cheng University, Taiwan, ROC
^fSchool of Civil Engineering and Surveying, University of Southern Queensland, Australia
^gDepartment of Biology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
^hDepartment of Environmental Sciences, COMSATS University Islamabad, Vehari 61100, Pakistan
ⁱUniversity of Wuppertal, School of Architecture and Civil Engineering, Institute of Foundation Engineering, Water, and Waste-Management, Laboratory of Soil, and Groundwater-Management, Pauluskirchstraße 7, 42285 Wuppertal, Germany
^jDepartment of Environmental and Energy Engineering, Yonsei University, Wonju 26493, South Korea
^kKing Abdulaziz University, Faculty of meteorology, Environment, and Arid Land Agriculture, Department of Arid Land Agriculture, Jeddah 21589, Saudi Arabia
^lUniversity of Kafrelsheikh, Faculty of Agriculture, Department of Soil and Water Sciences, 33516 Kafr El-Sheikh, Egypt

¹These authors contributed equally to this manuscript.
Corresponding authors: Sang Soo Lee, Sabry M. Shaheen, Pei Zhou

Abstract

Melatonin (MT) has recently gained significant scientific interest, though its mechanism of action in enhancing plant vigor, cadmium (Cd) tolerance, and Cd phytoremediation processes are poorly understood. Therefore, here we investigated the beneficial role of MT in improving growth and Cd remediation potential of rapeseed (Brassica napus). Plants, with or without MT (200 µM L−1), were subjected to Cd stress (30 mg kg1). Without MT, higher Cd accumulation (up to 99%) negatively affected plant growth and developmental feature as well as altered expression of several key genes (DEGs) involved in different molecular pathways of B. napus. As compared to only Cd-stressed counterparts, MT-treated plants exhibited better physiological performance as indicated by improved leaf photosynthetic and gaseous exchange processes (3–48%) followed by plant growth (up to 50%), fresh plant biomass (up to 45%), dry plant biomass (up to 32%), and growth tolerance indices (up to 50%) under Cd exposure. MT application enhanced Cd tolerance and phytoremediation capacity of B. napus by augmenting (1) Cd accumulation in plant tissues and its translocation to above-ground parts (by up to 45.0%), (2) Cd distribution in the leaf cell wall (by up to 42%), and (3) Cd detoxification by elevating phytochelatins (by up to 8%) and metallothioneins (by upto 14%) biosynthesis, in comparison to Cd-treated plants. MT played a protective role in stabilizing hydrogen peroxide and malondialdehyde levels in the tissue of the Cd-treated plants by enhancing the content of osmolytes (proline and total soluble protein) and activities of antioxidant enzymes (SOD, CAT, APX and GR). Transcriptomic analysis revealed that MT regulated 1809 differentially expressed genes (828 up and 981 down) together with 297 commonly expressed DEGs (CK vs Cd and Cd vs CdMT groups) involved in plant-pathogen interaction pathway, protein processing in the endoplasmic reticulum pathway, mitogen-activated protein kinase signaling pathway, and plant hormone signal transduction pathway which ultimately promoted plant growth and Cd remediation potential in the Cd-stressed plants. These results provide insights into the unexplored pleiotropic beneficial action of MT in enhancing in the growth and Cd phytoextraction potential of B. napus, paving the way for developing Cd-tolerant oilseed crops with higher remediation capacity as a bioecological trial for enhancing phytoremediation of hazardous toxic metals in the environment.

논문정보

형식Research article
게재일2023년 06월 (BRIC 등록일 2023-08-22)
연구진국내(교신)+국외 연구진
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