한빛사논문
- 조회501
Youseong Kim1, InHo Park2,3,4, Jiwoo Shin1, Jaibyung Choi1, Chansol Jeon5, Seonghun Jeon1, Jeon-Soo Shin2,3,4 and Hyungil Jung1,5*
1Department of Biotechnology, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 03722, Republic of KoreaI.
2Department of Microbiology, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seoul03722, Republic of KoreaI.
3Institute of Immunology and Immunological Diseases, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seoul03722, Republic of KoreaI.
4Severance Biomedical Science Institute, Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, 50-1 Yonsei-ro,Seoul03722, Republic of Korea
5JUVIC, 208Ho, 272, Digital-ro, Guro-gu, Seoul 08389, Republic of Korea
* Correspondence: HyungilJung, Ph. D.
Abstract
The coronavirus pandemic accelerates the development of next-generation vaccination technology to combat future pandemic outbreaks. Mucosal vaccination effectively protects the mucosal surfaces, the primary sites of viral entry, by inducing the secretion of immunoglobulin A (IgA) and humoral IgG. Here, w e adopted a dissolving microneedle (DMN) as a mucosal vaccine delivery platform to directly penetrate the sublingual site, which is rich in antigen-presenting cells (APCs) and lymphoid tissues. O ur sublingual dissolving microneedle (SLDMN) vaccination platform comprised a micropillar-based compartment and a 3D-printed SLDMN applicator as a substitute for the DMN patch. W e assessed the penetration efficacy of SLDMNs using in vitro optical coherence tomography (OCT) and in vivo histological analysis. W e also evaluated the efficacy of SLDMN in a vaccine form using the recombinant spike (S1) protein of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Furthermore, w e used SLDMN to challenge transgenic mice expressing human angiotensin-converting enzyme 2 (hACE2) receptors. W e evaluated its effects on antibody production, survival rate, and inflammation attenuation after infection compared to the intramuscular (IM) injections. Overall, SLDMN effectively induced mucosal immunity via IgA secretion, attenuated lung inflammation, and lowered the levels of cytokines and chemokines, which might prevent the “cytokine storm” after SARS-CoV-2 infection.
논문정보
- Research article
- 2023년 06월 (BRIC 등록일 2023-06-21)
- 국내 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
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