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조회432

한국생명공학연구원

J. Hazard. Mater., Available online 23 May 2023 | https://doi.org/10.1016/j.jhazmat.2023.131678 Three-dimensional label-free visualization of the interactions of PM2.5 with macrophages and epithelial cells using optical diffraction tomography

Wang Sik Lee ^a,1, Inha Kang ^b,1, Sung-Jin Yoon ^a, Hyunjung Kim^a, Yugyeong Sim ^a,c, Youngjun Park ^a, Jinah Park^b, Jinyoung Jeong^a,c

^aEnvironmental Disease Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu Daejeon, 34141, Republic of Korea
^bSchool of Computing, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu Daejeon, 34141, Republic of Korea
^cDepartment of Nanobiotechnology, KRIBB School of Biotechnology, University of Science and Technology (UST), 217 Gajeong-ro, Yuseong-gu Daejeon, 34113, Republic of Korea

¹These authors contributed equally.
Corresponding authors : Jinah Park, Jinyoung Jeong

Abstract

Particulate matter ≤ 2.5 µm (PM2.5) poses health risks related to various diseases and infections. However, the interactions between PM2.5 and cells such as uptake and cell responses have not been fully investigated despite advances in bioimaging techniques, because the heterogeneous morphology and composition of PM2.5 make it challenging to employ labeling techniques, such as fluorescence. In this work, we visualized the interaction between PM2.5 and cells using optical diffraction tomography (ODT), which provides quantitative phase images by refractive index distribution. Through ODT analysis, the interactions of PM2.5 with macrophages and epithelial cells, such as intracellular dynamics, uptake, and cellular behavior, were successfully visualized without labeling techniques. ODT analysis clearly shows the behavior of phagocytic macrophages and nonphagocytic epithelial cells for PM2.5. Moreover, ODT analysis could quantitatively compare the accumulation of PM2.5 inside the cells. PM2.5 uptake by macrophages increased substantially over time, but uptake by epithelial cells increased only marginally. Our findings indicate that ODT analysis is a promising alternative approach to visually and quantitatively understanding the interaction of PM2.5 with cells. Therefore, we expect ODT analysis to be employed to investigate the interactions of materials and cells that are difficult to label.

논문정보

형식Research article
게재일2023년 05월 (BRIC 등록일 2023-06-19)
연구진국내 연구진
분야 바이오·의료융합 > 바이오소재

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