한빛사논문
- 조회763
SeoYul Jo1,5, JiUn Lee1,5, Hyeongjin Lee1,2, Dongryeol Ryu3 and GeunHyung Kim1,4
1Department of Precision Medicine, Sungkyunkwan University School of Medicine, Suwon, Republic of Korea.
2Department of Biotechnology and Bioinformatics, Korea University, Sejong, Republic of Korea.
3Department of Biomedical Science and Engineering, Gwangju Institute of Science and Technology, Gwangju, Republic of Korea.
4Department of Biophysics, Institute of Quantum Biophysics, Sungkyunkwan University, Suwon, Republic of Korea.
5These authors contributed equally: SeoYul Jo, JiUn Lee.
Corresponding author : Correspondence to GeunHyung Kim.
Abstract
The fabrication of highly porous cell-loaded structures in tissue engineering applications has been a challenging issue because non-porous cell-laden struts can cause severe cell necrosis in the middle region owing to poor transport of nutrients and oxygen. In this study, we propose a versatile handheld 3D printer for the effective fabrication of porous cell-laden methacrylated gelatin (GelMa) with high porosity (≈97%) by air injection and a bubble-making system using mesh filters through which a mixture of air/GelMa bioink is passed. In particular, the pore size and foamability of the cell constructs could be manipulated using various processing parameters (rheological properties of GelMa, filter size and number, and air-bioink volume ratio). To demonstrate the feasibility of the cell construct as a tissue engineering substitute for muscle regeneration, in vitro cellular activities and in vivo regeneration ability of human adipose stem cells were assessed. The in vitro results demonstrated that the human adipose stem cells (hASCs) fabricated using the handheld 3D printer were alive and well-proliferated. Furthermore, the in vivo results showed that the hASCs-constructs directly printed from the handheld 3D printer showed significant restoration of functionality and efficient muscle regeneration in the volumetric muscle loss model of mice. Based on these results, the fabrication method of the porous cell-laden construct could be a promising tool for regenerating muscle tissues.
논문정보
- Research article
- 2023년 06월 (BRIC 등록일 2023-06-15)
- 국내 연구진
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