한빛사논문
- 조회462
Daryeon Son1,2,6, Jie Zheng1,2,6, In Yong Kim1,2,6, Phil Jun Kang1, Kyoungmin Park1, Lia Priscilla1, Wonjun Hong1, Byung Sun Yoon3, Gyuman Park4, Jeong-Eun Yoo4, Gwonhwa Song 2, Jang-Bo Lee5 and Seungkwon You1,2
1Laboratory of Cell Function Regulation, Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Republic of Korea.
2Institute of Animal Molecular Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Republic of Korea.
3Institute of Regenerative Medicine, STEMLAB, Inc., Seoul 02841, Republic of Korea.
4Institute of Future Medicine, STEMLAB, Inc., Seoul 02841, Republic of Korea.
5Department of Neurosurgery, College of Medicine, Korea University Anam Hospital, Seoul 02841, Republic of Korea.
6These authors contributed equally: Daryeon Son, Jie Zheng, In Yong Kim.
Corresponding authors : Correspondence to Gwonhwa Song, Jang-Bo Lee or Seungkwon You.
Abstract
Spinal cord injury (SCI) is a clinical condition that leads to permanent and/or progressive disabilities of sensory, motor, and autonomic functions. Unfortunately, no medical standard of care for SCI exists to reverse the damage. Here, we assessed the effects of induced neural stem cells (iNSCs) directly converted from human urine cells (UCs) in SCI rat models. We successfully generated iNSCs from human UCs, commercial fibroblasts, and patient-derived fibroblasts. These iNSCs expressed various neural stem cell markers and differentiated into diverse neuronal and glial cell types. When transplanted into injured spinal cords, UC-derived iNSCs survived, engrafted, and expressed neuronal and glial markers. Large numbers of axons extended from grafts over long distances, leading to connections between host and graft neurons at 8 weeks post-transplantation with significant improvement of locomotor function. This study suggests that iNSCs have biomedical applications for disease modeling and constitute an alternative transplantation strategy as a personalized cell source for neural regeneration in several spinal cord diseases.
논문정보
- Research article
- 2023년 06월 (BRIC 등록일 2023-06-15)
- 국내 연구진
- 생명과학 > 발생생물학
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