한빛사논문
- 조회672
Joon-Ha Park 1, Chi-Pin James Wang 2,3, Hye-Jin Lee 4, Kyung Soo Hong 5, Jung Hong Ahn 5, Yeon-Woo Cho 1, Jeong-Hyeon Lee 1, Hee Seung Seo 2,3, Wooram Park 6, Se-Na Kim 7,8, Chun Gwon Park 2,3,9, Wonhwa Lee 4, Tae-Hyung Kim 1
1School of Integrative Engineering, Chung-Ang University, 06974, Seoul, Republic of Korea.
2Department of Biomedical Engineering, SKKU Institute for Convergence, Sungkyunkwan University (SKKU), 16419, Suwon, Republic of Korea.
3Department of Intelligent Precision Healthcare Convergence, SKKU Institute for Convergence, Sungkyunkwan University (SKKU), 16419, Suwon, Republic of Korea.
4Department of Chemistry, Sungkyunkwan University, 16419, Suwon, Republic of Korea.
5Division of Pulmonology and Allergy, Department of Internal Medicine, College of Medicine, Yeungnam University, Regional Center for Respiratory Diseases, Yeungnam University Medical Center, 42415, Daegu, Republic of Korea.
6Department of Integrative Biotechnology, College of Biotechnology and Bioengineering, Sungkyunkwan University, Seoburo 2066, Suwon, Gyeonggi, 16419, Republic of Korea.
7Research and Development Center, MediArk Inc., Cheongju, Chungbuk, 28644, Republic of Korea.
8Department of Industrial Cosmetic Science, College of Bio-Health University System, Chungbuk National University, Cheongju, Chungbuk, 28644, Republic of Korea.
9Biomedical Institute for Convergence at SKKU (BICS), Sungkyunkwan University, Suwon, 16419, Republic of Korea.
J.-H.P., C.-P.J.W., and H.-J.L. contributed equally to this work.
CORRESPONDING AUTHORS : Chun Gwon Park, Wonhwa Lee, Tae-Hyung Kim
Abstract
Deoxyribonuclease-I (DNase-I), a representative endonuclease, is an important biomarker for the diagnosis of infectious diseases and cancer progression. However, enzymatic activity decreases rapidly ex vivo, which highlights the need for precise on-site detection of DNase-I. Here, a localized surface plasmon resonance (LSPR) biosensor that enables the simple and rapid detection of DNase-I is reported. Moreover, a novel technique named electrochemical deposition and mild thermal annealing (EDMIT) is applied to overcome signal variations. By taking advantage of the low adhesion of gold clusters on indium tin oxide substrates, both the uniformity and sphericity of gold nanoparticles are increased under mild thermal annealing conditions via coalescence and Ostwald ripening. This ultimately results in an approximately 15-fold decrease in LSPR signal variations. The linear range of the fabricated sensor is 20-1000 ng mL-1 with a limit of detection (LOD) of 127.25 pg mL-1 , as demonstrated by spectral absorbance analyses. The fabricated LSPR sensor stably measured DNase-I concentrations from samples collected from both an inflammatory bowel disease (IBD) mouse model, as well as human patients with severe COVID-19 symptoms. Therefore, the proposed LSPR sensor fabricated via the EDMIT method can be used for early diagnosis of other infectious diseases.
논문정보
- Research article
- 2023년 06월 (BRIC 등록일 2023-06-12)
- 국내 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
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