한빛사논문
- 조회556
Hyo Lee 1, Sojeong Lee 1, Chaewon Park 1, Minjoo Yeom 2, Jong-Woo Lim 2, Thi Thu Hang Vu 3, Eunjung Kim 4,5, Daesub Song 2, Seungjoo Haam 1
1Department of Chemical and Biomolecular Engineering, Yonsei University, Seoul, 03722, Republic of Korea.
2Department of Veterinary Medicine Virology Laboratory, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, 08826, Republic of Korea.
3Department of Preclinical Science, College of Pharmacy, Korea University Sejong Campus, Sejong City, 30019, Republic of Korea.
4Division of Bioengineering, Incheon National University, Incheon, 22012, Republic of Korea.
5Department of Bioengineering & Nano-Bioengineering, Research Center for Bio Materials and Process Development, Incheon National University, Incheon, 22012, Republic of Korea.
H.L. and S.L. contributed equally to this work.
CORRESPONDING AUTHORS: Eunjung Kim, Daesub Song, Seungjoo Haam
Abstract
African swine fever virus (ASFV) is a severe and persistent threat to the global swine industry. As there are no vaccines against ASFV, there is an immense need to develop easy-to-use, cost-effective, and rapid point-of-care (POC) diagnostic platforms to detect and prevent ASFV outbreaks. Here, a novel POC diagnostic system based on affinity column chromatography for the optical detection of ASFV is presented. This system employs an on-particle hairpin chain reaction to sensitize magnetic nanoclusters with long DNA strands in a target-selective manner, which is subsequently fed into a column chromatography device to produce quantitatively readable and colorimetric signals. The detection approach does not require expensive analytical apparatus or immobile instrumentation. The system can detect five genes constituting the ASFV whole genome with a detection limit of ≈19.8 pm in swine serum within 30 min at laboratory room temperature. With an additional pre-amplification step using polymerase chain reaction (PCR), the assay is successfully applied to detect the presence of ASFV in 30 suspected swine samples with 100% sensitivity and specificity, similar to quantitative PCR. Thus, this simple, inexpensive, portable, robust, and customizable platform for the early detection of ASFV can facilitate the timely surveillance and implementation of control measures.
논문정보
- Research article
- 2023년 03월 (BRIC 등록일 2023-06-14)
- 국내 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
댓글 작성 로그인
팝업 닫기관련 링크
연구자 키워드
연구자 ID
관련분야 연구자보기
관련분야 논문보기
